中文摘要：

采用表面引发原子转移自由基聚合（ATRP）的方法制备了聚（N-异丙基丙烯酰胺）（PNIPAAm）改性表面，通过改变聚合时间进而调控表面接枝层厚度。水接触角和蛋白质吸附测试发现PNIPAAm改性表面的浸润性和对蛋白质吸附都具有一定的温度响应性，且随着PNIPAAm接枝层厚度的增加，该温度响应性有一定程度的增强。同时，纤维蛋白原和溶菌酶的吸附测试表明PNIPAAm改性表面对尺寸不同的蛋白质具有不同的温度响应性。

英文摘要：

The poly（N-isopropylacrylamide） （PNIPAAm） brushes are prepared via surface-initiated atom transfer radical polymerization （SI-ATRP） on initiator-immobilized silicon surfaces. The thickness of grafted PNIPAAm layer is modulated by adjusting polymerization time. The PNIPAAm modified surface exhibits thermo-re- sponsivity to both wettability and protein adsorption in some extent and the thermo-responsivity is enhanced as the thickness of PNIPAAm layer increases. Moreover, the adsorption results of two proteins（fibrinogen and lysozyme） with different sizes indicate that the PNIPAAm-modified surface exhibits a size-sensitive property of protein adsorption.