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斑透翅蝉唾液腺细菌群落组成研究

ISSN号：0253-2654
期刊名称：《微生物学通报》
时间：0
分类：Q958.15[生物学—动物学]
作者机构：[1]西北农林科技大学植物保护学院植物资源保护与害虫防治教育部重点实验室,陕西杨凌712100, [2]西北农林科技大学林学院,陕西杨凌712100
相关基金：The National Natural Science Foundation of China（No.31093430,31170360）; Program for New Century Excellent Talents in Universities of China（No.NCET-10-0691）

关键词：斑透翅蝉, 唾液腺, 细菌群落, 绿脓假单胞杆菌, 肠杆菌属, Hyalessa maculaticollis, Salivary glands, Microbiota, Pseudomonas aeruginosa, Enterobacter

中文摘要：

【目的】为明晰蝉类昆虫唾液腺中细菌的组成及其中是否存在内共生菌Candidatus Sulcia muelleri。【方法】以斑透翅蝉Hyalessa maculaticollis（Motschulsky）为材料,采用16S rRNA限制性内切酶片段长度多态性（RFLP）对其唾液腺细菌群落组成进行分析。【结果】斑透翅蝉唾液腺中共存在7种细菌,分别属于变形菌门和厚壁菌门;其中绿脓假单胞杆菌Pseudomonas aeruginosa和肠杆菌Enterobacter sp.为优势细菌,分别占克隆总数的48.7%;另外5种细菌（反硝化细菌热单胞菌Thermomonas brevis、鞘胺醇单胞菌Sphingomonas sp.、芽孢杆菌Bacillus sp.、厌氧球菌Anaerococcus sp.和Methyloversatilis sp.）总共占克隆文库的2.05%。【结论】首次采用分子生物学方法明晰斑透翅蝉唾液腺的细菌群落;其细菌群落组成相对简单,且两种细菌占主导地位;此外,头喙亚目昆虫体内特有的内共生菌Candidatus Sulcia muelleri未在斑透翅蝉唾液腺中检测出,表明该共细菌可能仅在腹部的贮菌体中分布;斑透翅蝉唾液腺中的细菌是否普遍存在于蝉科昆虫唾液腺中以及在取食韧皮部汁液过程中的功能有待进一步研究。

英文摘要：

[Objective] This paper aims to clarify the bacteria associated with the salivary glands of cicada Hyalessa maculaticollis（Motschulsky）,and to address if the endosymbiont Candidatus Sulcia muelleri occurrs in the salivary glands.[Methods] We investigated the bacterial community in the salivary glands of cicada H.maculaticollis using 16 S rRNA restriction fragment-length polymorphism（RFLP）.[Results] We identified seven bacteria in the salivary glands,which belong to the Phyla Proteobacteria and Firmicutes.The bacterial community is dominated by Pseudomonas aeruginosa and Enterobacter sp.,each of them accounted for 48.7% in the clone library.In total,the other five bacterial species（Thermomonas brevis,Sphingomonas sp.,Methyloversatilis sp.,Anaerococcus sp.and Bacillus sp.） together accounted for 2.05% in the clone library.[Conclusion] The results show that the bacterial species composition in the cicada salivary glands of H.maculaticollis was relatively simple,which were dominated by two bacterial species.The endosymbiont Candidatus Sulcia muelleri of Auchenorrhyncha,formerly detected from bacteriomes of related auchenorrhynchan hosts,were not detected in the salivary glands of H.maculaticollis.The bacteria harbored in salivary glands of cicadas probably play an important role in both food ingestion and interactions between the cicadas and their host plants.However,whether the related bacteria are commonly harbored in other cicadas and their certain function need to be further investigated.