中文摘要：

研究含黄连的不同复方水提液体系经大孔吸附树脂精制后,黄连中主要指标性成分生物碱的变化,阐明不同复方组合-配伍对药方中组成药味指标性成分的影响,为大孔树脂精制技术在中药复方中的应用提供实验依据。选用黄连用量和复方总用量均相同、组方药味不同的复方1～6。通过考察5种树脂AB-8、S-8、X-5、NKA-Ⅱ、NKA-9对黄连中盐酸小檗碱的静态吸附率,确定AB-8树脂作为复方1～6吸附动力学比较研究的树脂;通过比较复方1～6水提液体系在相同树脂环境下的静态吸附过程,考察6种不同复方组成药味对复方中指标性成分分离纯化的影响。实验结果表明,由于体系本身溶液环境的差异,复方1～6各指标性成分达到吸附平衡的时间均有差异。AB-8树脂对复方1～6水提液体系的静态吸附在开始阶段吸附量的增加比较明显,在40min后,由于吸附竞争点饱和的原因,吸附量的增加均比较平稳;黄连与不同药材配伍时,虽然配伍药材所含的化合物类型差异较大,但黄连中生物碱类成分均可以在树脂上被选择性吸附,可知在复方水提液复杂体系中可以选择性地分离纯化生物碱类有效成分。

英文摘要：

To study the different TCM Compounds systems that extracted by water and be composed of Rhizoma Coptidis and other cooperated medicines. The water systems were refined by macroporous resin and the changed contents of alkaloid detected to clarify the effects in different TCM Compounds. The study provide experimental basis on using of macroporous resin purification technology in TCM Compounds. Six TCM compounds involved the same Rhizoma Coptidis dose and total dose were chosen and were extracted by water to produce six water systems. First, AB-8 resin was chosen in the next study through the detection of adsorption rate with berberine on five kinds of resin AB-8, S-8, X-5, NKA-Ⅱand NKA-9. Secondly, a comparative study of adsorption kinetics of these six TCM compounds on AB-8 resin by determined the changed contents of alkaloid. The results showed that the time of equiUbrium adsorption represented by alkaloids were varied due to the different solution environment and the adsorption capacity on AB-8 resin was increased obviously in the initial stage, but the capacity increased smoothly after 40 min because of the saturation. Meanwhile, the alkaloids derived from Rhizoma Coptidis in different TCM compounds always can be selectively purified by macroporous resin.