中文摘要：

目的研究二烯丙三硫（DATS）对乳腺癌MDA—MB-231细胞增殖和侵袭能力的影响及对尿激酶型纤溶酶激活物（uPA）系统的调节作用。方法实时定量PCR法（real—timePCR）检测乳腺癌细胞株uPA系统中uPA、uPA受体（uPAR）和uPA抑制物（PAI-1）的表达；使用不同浓度DATS处理乳腺癌细胞，MTS比色法测定细胞的增殖能力；Matrigel体外浸润实验检测DATS（20～60μmol/L）对细胞浸润能力的影响；Transwell小室迁移实验和划痕试验评价60μmol／LDATS对细胞迁移能力的影响；RT—PCR和Westernblot分别测定60txmol／LDATS作用后，乳腺癌细胞中uPA、uPAR和PAI-1mRNA和蛋白表达的变化；ELISA法检测细胞培养液中PAI-1蛋白含量的变化。结果20～80μmol／LDATS对高侵袭性乳腺癌细胞株MDA—MB-231的生长有明显的抑制作用，且呈浓度和时间依赖性。60μmol／LDATS明显抑制MDA-MB-231细胞的浸润和迁移能力，并在mRNA和蛋白水平上，显著上调PAI-1的表达，但对uPA和uPAR的表达水平无显著影响。结论DATS抑制MDA-MB-231细胞的增殖和侵袭能力，并在mRNA和蛋白水平上，显著上调PAI-1的表达。

英文摘要：

Objective To investigate the effects of diallyl trisulfide （DATS） on proliferation and invasion of breast cancer MDA-MB-231 cells and the role for DATS in regulation of urokinase plasminogen activator （uPA） system molecules. Methods Real-time PCR was used to detect mRNA expressions of uPA, uPA receptor （uPAR） and uPA inhib- itor-1 （PAI-1） in breast cancer cell lines. MDA-MB-231 cells were treated with various concentrations of DATS, fol- lowed by MTS assay to determine cellular proliferation. Martrigel invasion assays were performed to determine invasive capacity of cancer cells after 20-60 ixmol/L DATS treatments. Transwell motility assay and healing assay were utilized to detect the effect of 60 ixmol/L DATS on migration of MDA-MB-231 ceils. Real time -PCR and western blot were used to detect mRNA and protein expressions of nPA, uPAR and PAI-1 in tumor cells. Concentrations of PAI-1 protein were determined by ELISA assay in conditioned medium with DATS treatment. Results 20-80 μmol/L DATS signifi- cantly inhibited proliferation of highly invasive breast cancer MDA-MB-231 cells in a dose- and time-dependent manner. 60μmol/L DATS inhibited invasiveness and motility capacity of MDA-MB-231 cells. PAI-1 expression was significantlyincreased by 60 ixmoL/L DATS at mRNA and protein levels, whereas uPA and uPAR expressions were not. Conclusion DATS significantly inhibits proliferation and invasion of MDA-MB-231 cell line and up-regulates PAI-1 expression at mRNA and protein levels.