采用形态特征观察及16S rDNA 序列分析等方法,对分离自健康水稻植株的内生链霉菌菌株F-1 进行了鉴定;采用对峙培养试验测定了菌株F-1 对水稻纹枯病菌(Rhizoctonia solani)的拮抗作用,在离体水稻叶片和活体水稻植株上,测定了菌株F-1 代谢产物对水稻纹枯病的防治效果.结果表明:菌株F-1 与普特拉链霉菌(Streptomyces platensi)亲缘关系最近,同源性达100%,且形态与培养特征、生理生化特性与普特拉链霉菌相符,故将其命名为普特拉链霉菌(Streptomyces platensi)F-1;其对水稻纹枯病菌有显著的拮抗作用,其代谢产物导致水稻纹枯病菌顶端菌丝弯曲,菌丝体皱缩畸形;在离体叶片和活体水稻植株上,菌株F-1 的培养物滤液能够有效防治水稻纹枯病.普特拉链霉菌菌株F-1 对水稻纹枯病具有较好的生防潜力.
Strain F-1 was identified by morphological and cultural traits, physio-biochemical characteristics and 16S rDNA sequence analysis. Antagonistic interaction between strain F-1 and Rhizoctonia solani, was tested using the dual cultural method. Cultural filtrate of strain F-1 was determined for control of R. solani on in vitro rice leaves and on rice plants. The results showed that strain F-1 was most closely related to Streptomyces plateusis (100% si milarity) by the 16S rDNA sequence analysis, the morphological and cultural traits, and physio-biochemical characte ristics of strain F-1 conformed with S. Platensis, so strain F-1 was affiliated to Streptomyces platensis F-1. It inhib ited growth of R. solani, resulting in hyphal malformation. Application of the culture filtrate of strain F-1 to rice d etached rice leaves and to potted rice plants reduced disease incidence caused by R. solani effectively. Strain F-lw as preliminarily identified as S. platensis F-l, and it is an effective agent against R. solani.