中文摘要：

目的探讨体外内皮细胞膜微粒（endothelial microparticle,EMPs）对血管内皮细胞凋亡功能的影响。方法将内皮细胞自身产生的膜微粒与内皮细胞共培养,评估共培养下内皮细胞凋亡的变化。结果 7.66×10~4,7.66×10~3,7.66×10~2个/ml浓度的EMPs组（简称高、中、低浓度组）其内皮细胞caspase-3活性、磷脂酰丝氨酸（Phosphatidylserine,PS）活性表达均高于0个/ml浓度EMPs的对照组（P〈0.05）。PCR检测凋亡基因变化后,发现高、中浓度EMPs组APAF-1（apoptotic protease activating factor-1）基因表达较对照增高（P=0.003、P=0.000）,低浓度组没有明显差异（P=0.272）,AIF（apoptosis-inducing factor）基因方面,以Realtime-PCR方法测定,RQ值代表的是各组/对照组的基因表达量,则提示高中浓度EMPs组AIF表达较对照增高（RQ=1.836、RQ=1.324）,低浓度组则呈现下降（RQ=0.569）。结论内皮细胞膜微粒在体外可促进血管内皮细胞凋亡发生。7.66×10~4、7.66×10~3、7.66×10~2个/ml浓度EMPs可促进细胞Caspase-3、PS表达活性增强,7.66×10~4、7.66×10~3个/ml浓度EMPs可促进APAF-1、AIF凋亡基因表达,7.66×10~2个/ml浓度无明显促进作用。

英文摘要：

Objective To investigate the effects of EMPs on HUVECs apoptosis in vitro.Methods We incubated HUVECs with EMPs released by HUVECs,then estimated the endothelial apoptosis.Results 7.66×10~4,7.66×10~3, 7.66×10~2 /ml concentrations of EMPs（high,moderate,low concentration groups） induced higher activity of Caspase-3 and PS than the control group.After apoptosis genes analyzed by PCR,we found moderate and high concentration of EMPs increased expression of APAF-1 gene in comparison with control group（P= 0.003,P = 0.000）,while low concentration group had no difference compared to control group（P= 0.272 ）.We used Realtime-PCR and the RQ value to measure AIF gene expression in experimental and control group.It is indicated that moderate and high concentration groups increased expression of AIF gene in comparison with control group（RQ =1.836,RQ =1.324）,while low concentration group showed decreased value（RQ =0.569）.Conclusion EMPs can induce HUVECS apoptosis progress in vitro.7.66×10~4,7.66×10~3,7.66×10~2/ml concentrations of EMPs induced increased activity of Caspase-3 and PS.7.66×10~4, 7.66×10~3/ml concentrations of EMPs induced APAF-1 and AIF gene expression,while 7.66×10~2/ml concentrations of EMPs has no such effect.