中文摘要：

目的研究补骨脂素（psoralen,Pso）对人类乳腺癌细胞株增殖的影响。方法采用噻唑蓝（MTT）比色法测定补骨脂素对雌激素依赖性乳腺癌细胞MCF-7和非雌激素依赖性乳腺癌细胞MDA-MB231的细胞增殖作用,并以雌激素受体拮抗剂ICI182,780为工具药来评价补骨脂素发挥雌激素样作用与雌激素受体的关系,流式细胞术对MCF-7细胞的增殖情况进行分析。结果与溶剂对照组相比较,Pso（10～40μmol.L^-1）能明显促进MCF-7细胞的增殖,而对雌激素受体阴性MDA-MB231细胞未见影响,并将MCF-7细胞周期由G1期向S期推进,促进DNA合成,提高细胞分裂增殖指数,且Pso促进MCF-7细胞增殖作用被雌激素受体拮抗剂所拮抗。结论补骨脂素具有雌激素活性,此作用是通过雌激素受体（ER）介导的。

英文摘要：

Aim To investigate the effects of psoralen on the proliferation of human breast carcinoma cells in vitro.Methods Effects of psoralen on the cell proliferation was tested in ER-positive MCF-7 cell and ER-negative MDA-MB231cell by MTT assay.The estrogen-like effect of psoralen and its relation with the estrogen-receptor were evaluated by using pure estrogen receptor antagonist ICI182,780 as a tool.Proliferation of MCF-7 cell was examined by flow cytometer.Results Psoralen（10～40 μmol·L^-1）stimulated proliferation of ER-positive MCF-7 cell compared with solvent control,whereas effect on ER-negative MDA-MB231 cell proliferation was not found,the cell cycle was impulsed from G1 to S,DNA synthesizing was enhanced,and PI also increased.The above function of boosting MCF-7 cell proliferation could be inhibited by adding estrogen receptor antagonist ICI182,780.Conclusions Psoralen has the estrogen-like activities through the estrogen response pathway.