选用Middle Fine Sephadex G-150(MFG-150)、Fine Sephadex G-150(FG-150)以及Sephacryl S-300(S-300)3种凝胶过滤对富含R-藻红蛋白(R-PE)的多管藻藻胆蛋白提取液进行R-PE、R-藻蓝蛋白(R-PC)和别藻蓝蛋白(AP)的分离,并对所得R-PE、R-PC/AP组分用DEAE Sepharose-Fast Flow离子交换层析做进一步纯化,优化建立了从多管藻藻胆蛋白提取液中同时有效分离纯化R-PE、R-PC和AP3类藻胆蛋白组分的技术方法.
R-phycerythrin (R-PE), R-phycocyanin (R-PC) and allophycocyanin (AP) are isolated from the extrac- ted phycobiliprotein solution of Polysiphonia urceolata rich in phycoerythrin using gel filtration through Middle Fine Sephadex G-150 (MFG-150), Fine Sephadex G-150(FG-150) and Sephaeryl S-300(S-300). The obtained fractions of R-PE, R-PC and AP are further purified using ion-exchange chromatography through DEAE Sepharose-Fast Flow. An optimized technical procedure for simultaneous preparation of the three phycobiliprotein types from the extracted phycobiliproteins of Polysiphonia urceolata is established based on the present experiments.