研究了平榛(Corylus heterophylla Fisch)正常胚珠与败育胚珠在果实发育期问4种植物内源激素的变化动态,并使用ABA与其合成抑制剂钨酸钠对果序进行喷施处理,研究其对胚珠发育的影响。结果表明:(1)在正常充实胚珠中,IAA、GA3与ZR含量均大致呈单峰曲线,峰值分别出现在花后80、90和100d,同期相比,败育胚珠中IAA、GA3多数低于前者,而ZR含量多数高于前者;花后80~110d是胚珠快速生长期,在此期间,正常发育胚珠中ABA的含量显著高于败育胚珠;(2)与对照相比,50.0和20.0mg·L^-1的ABA处理不同程度的提高了果实种仁质量、果壳质量及果实总质量等,而ABA合成抑制剂钨酸钠处理效果与ABA相反,表明ABA参与调控了榛子胚珠充实发育的进程。
Changing dynamics of four endogenous hormones in normal and aborted ovules of hazelnut (Corylus heterophylla Fisch) was analyzed during fruit development stage, and following ABA and its synthesis inhibitor sodium tungstate solution spraying treatments were carded out to study the effects of ABA on ovules filling process. The results showed that: ( 1 ) Content changing curves of IAA, GA3 and ZR in normal filled ovules were approximately single peak shaped, and their peak value appeared on 80th, 90th and 100th day after blooming, respectively. Compared during the same developing period, content of IAA and GA3 in aborted ovules were lower than that in filled ovules in most cases, while higher ZR content was found to exist in aborted ovules. Eighty to 110 days after blooming was a key period for rapid growth of hazelnut ovule. During the stage, ABA content in developing ovules was significantly higher than that in abortive one. (2) 20.0 and 50.0 mg ·L-1 ABA treatments promoted fresh weight of kernel, shell and nut to different degrees, while sodium tungstate had an opposite effects on the above indexes compared with ABA treatments. The results above indicated that ABA was involved in the regulation process of ovule filling in hazelnut.