中文摘要：

目的：通过四氢吡咯二硫代氨基甲酯（PDTC）研究核因子-κB（NF-κB）对人脐静脉内皮细胞模拟缺血再灌注后趋化因子CXCL16表达的影响。方法：分为5组对人脐静脉内皮细胞进行培养,即对照组（正常培养基培养）、缺血再灌注组（模拟缺血培养液培养30min后换正常培养液再培养4h）、缺血再灌注＋0.1mmol/L PDTC组、缺血再灌注＋0.25mmol/L PDTC组和缺血再灌注＋0.5mmol/L PDTC组。后3组均于模拟缺血再灌注培养前1h在培养液中添加相应浓度PDTC。观察各组NF-κB p65及CXCL16mRNA表达水平,通过ELISA检测CXCL16蛋白表达水平以及细胞存活情况。结果：缺血再灌注组显著刺激CXCL16的mRNA和蛋白表达水平（均P〈0.05）。0.1mmol/L、0.25mmol/L和0.5mmol/L PDTC均显著降低NF-κB mRNA和CXCL16mRNA（均P〈0.05）。0.5mmol/L PDTC显著降低上清培养液CXCL16蛋白表达水平（P〈0.05）。0.1mmol/L、0.25mmol/L和0.5mmol/L PDTC均促进细胞存活（均P〈0.05）。结论：PDTC抑制模拟缺血再灌注所诱导的CXCL16表达,有利于细胞生存。

英文摘要：

Objective： To investigate the regulation mechanism of CXCL16 after mimic ischemia/reperfusion culture of human umbilical vein endothelial cells （HUVECs） with the utilization of PDTC. Method： HUVECs were divided into 5 groups： control group （culture with normal media）, mimic ischemia/reperfusion group （mimic is- chemic media cultured for 30 min followed by normal media cultured for 4 h）, mimic ischemia/reperfusion group is＋0. 1 m mol/L PDTC group, mimic ischemia/reperfusion group ＋0.25 m mol/L PDTC group and mimic ische- mia/reperfusion group ＋0.5 m mol/L PDTC group. For the latter 3 groups, HUVECs were treated with PDTC 1 h before mimic ischemia/reperfusion. The inhibition efficiency of PDTC on the mRNA and protein levels of p65, the expression of CXCLI6 mRNA and protein as well as cell survival were determined by real-time PCR, ELISA and WST assay respectively. Result：The mRNA and protein levels of CXCL16 were significantly increased by mim- ic ischemia/reperfusion （both P〈0.05）. 0.1,0.25 and 0.5 mmol/L PDTC significantly decreased mRNA levels of NF-κB and CXCL16 （both P〈0.05）. 0.5 mmol/L PDTC significantly suppressed supernatant CXCL16 protein level （P〈0.05）. 0.1, 0.25 and 0.5 mmol/L PDTC promoted cell survival （all P〈0.05）. Conclusion：PDTC inhibited mimic ischemic/reperfusion-induced expression of CXCL16 and promoted the survival of HUVECs.