研究了不同Cd2+浓度与硝酸盐浓度、磷酸盐浓度交互作用对杜氏盐藻(Dunaliellasalina)生长及叶绿素荧光特性的影响。结果表明,Cd2+浓度与硝酸盐浓度、磷酸盐浓度对杜氏盐藻的光合作用及生长均具有显著影响(P〈O.05);Cd2+胁迫24h后,杜氏盐藻的荧光参数Fv/FmFv/Fo、Yield、rETR、细胞密度和叶绿素相对含量均与Cd2+浓度表现为显著的负相关;在低硝酸盐摩尔浓度(55μmol/I.)下,杜氏盐藻的荧光参数Fv/Fm、Fv/Fo、Yield、rETR、细胞密度和叶绿素相对含量均明显下降;在高硝酸盐摩尔浓度(7040μmol/L)下,杜氏盐藻的上述荧光参数下降幅度小;在正常硝酸盐摩尔浓度(880/Lmol/L)下,杜氏盐藻荧光参数Fv/Fo、NPQ和叶绿素相对含量均最高,对Cd2+的耐受性更强;在低磷酸盐摩尔浓度(2.27μtool/L)下,杜氏盐藻的荧光参数(除NPQ外)、细胞密度和叶绿素相对含量均显著低于对照组和高磷处理组,表现为磷限制现象;在高磷酸盐摩尔浓度(290.40μmol/L)下,杜氏盐藻的荧光参数(除NPQ外)、细胞密度和叶绿素相对含量均明显高于低磷处理组和对照组,杜氏盐藻受Cd2+胁迫程度最小。
The interaction effects of Cd2+ , nitriate and phosphate concentration on the growth and chlorophyll fluorescence charteristics of Dunaliella salina were investigated in this paper. The results indicated that Cd2+ , nitri- ate and phosphate concentration had significant effects on the photosynthesis and growth of Dunaliella salina (P〈0.05). The fluorescence parameters of Fv/Fm, Fv/Fo, Yield and rETR, cell density and relative chlorophyll content of Dunaliella salina were significant negative correlated with Cd2+ concentrations after 24 h. And the fluorescence pa~ rameters evidently decreased in Dunaliella salina under low nitriate concentration of 55 μmol/L, and it also caused the decrease of the cell density and relative chlorophyll content. Under high nitriate concentration (7 040 μmol/L), there was no significant difference in the chlorophyll fluorescence parameters. The fluorescence parameters (Fv/Fo, NPQ), cell density and relative chlorophyll content of Dunaliella salina got to the greatest values at normal nitriate concentration of 880 μmol/L, and the Dunaliella salina had more tolerance to Cd2+. The fluorescence parameters (except NPQ), cell density and relative chlorophyll content of Dunaliella salina remarkably decreased in Dunaliella salina under low phosphate concentration (2.27 μmol/L), which showed obvious phosphorus limitation. The chloro- phyll fluorescence parameters, cell density and relative chlorophyll content reached the highest values at high phos- phate of 290.40 μmol/L, and the Cd2+ stress to Dunaliella salina was lowest,