中文摘要：

以大兴安岭地区野生蓝莓苗木为试材,取当年生幼嫩茎段为外植体,探索建立蓝莓组培苗的快繁体系。结果表明：外植体消毒处理组合为75%乙醇处理10-15s,0.1%升汞处理10min。培养基为WPM培养基,愈伤组织诱导及不定芽增殖时期应用的激素组合ZT 2.0mg/L＋NAA 0.2mg/L。利用IBA蘸根法诱导不定根,激素浓度为200μg/mL,芽诱导率超过80%,生根率超过70%。

英文摘要：

Ihking the wild blueberry seedlings in Greater hinggan mountains area as test materials,using the young stem segments as explants,the blueberry somaclone of rapid propagation system was explored. The results showed the explants disinfection treatment combination was 75% ethanol for 10--15 seconds and 0. 1% mercuric chloride for 10 min. The medium was WPM,and the hormones were ZT 2. 0 mg/L＋NAA 0. 2 mg/mL in the steps of inducement of callus and proliferation of adventitious buds. Adventitious roots were induced with dipping method hy 200 μg/ml. IBA. The results showed that callus and adventitious buds could be formed from stem of blueberry by the medium and hormones,and the induced ratio of adventitious buds was higher than 80%. In addition,the rooting rate was more than 70%.