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Sequencing and comparative analyses of Aegilops tauschii chromosome arm 3DS reveal rapid evolution of Triticeae genomes
  • ISSN号:1673-8527
  • 期刊名称:《遗传学报:英文版》
  • 时间:0
  • 分类:Q[生物学]
  • 作者机构:[1]State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China, [2]USDA-ARS West Regional Research Center, Albany, CA 94770, USA, [3]Department of Plant Sciences, University of California at Davis, Davis, CA 95616, USA, [4]Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA, [5]Arizona Genomics Institute, School of Plant Science, University of Arizona, Tucson, AZ 85721, USA
  • 相关基金:Acknowledgments The authors would like to thank james Thomson and Toni Mohr for their critical reading and providing comments to improve the manuscript. The work was supported by funding from the National Natural Science Foundation of China (Nos. 31290210, 31210103902), the Unites States National Science Foundation grant (No. IOS 1238231), the USDA-Agricultural Research Service CRIS project (No. 5325-21000-019), and the Ministry of Education of China (111 project).
中文摘要:

面包小麦(Triticum aestivum, AABBDD ) allohexaploid 种从二轮种间的杂交被导出。双 Aegilops tauschii 的一个高质量的染色体顺序集会,小麦 D 染色体的施主,将提供一个有用平台学习多倍体小麦进化。分享的用绳子拖的平底渡船和下一代的定序技术的一条联合途径被采用从 Ae 的短手臂定序 3176 用绳子拖的平底渡船克隆的最小的并列路径(MTP ) 。tauschii 染色体 3 (At3DS ) 。最后的集会 135 与 4.2 Mb 的 N50 超级支架被用来与 2222 预言的编码蛋白质的基因的一个总数造 247-Mb pseudomolecule。与米饭, Brachypodium,和蜀黍的 orthologous 区域相比, At3DS 包含 38.67% 更多的基因。与 At3DS 相比,小麦染色体 3B (Ta3BS ) 的短手臂顺序是在尺寸大的 95-Mb,它主要由于 non-centromeric 区域的扩大,建议在 Ta3B 的 transposable 元素(TE ) 爆炸多半发生在那里。另外,尺寸增加被 Ta3BS 的基因数字的比例的增加伴随。我们发现在小麦染色体 3D (Ta3DS ) 的短手臂的顺序,仅仅与 At3DS 相比有不到 0.27% 基因损失。我们的学习揭示草染色体的分叉的进化并且在多倍体小麦染色体提供新卓见进顺序变化。

英文摘要:

Bread wheat (Triticum aestivum, AABBDD) is an allohexaploid species derived from two rounds of interspecific hybridizations. A high-quality genome sequence assembly of diploid Aegilops tauschii, the donor of the wheat D genome, will provide a useful platform to study polyploid wheat evolution. A combined approach of BAC pooling and next-generation sequencing technology was employed to sequence the minimum tiling path (MTP) of 3176 BAC clones from the short arm ofAe. tauschii chromosome 3 (At3DS). The final assembly of 135 super-scaffolds with an N50 of 4.2 Mb was used to build a 247-Mb pseudomolecule with a total of 2222 predicted protein-coding genes. Compared with the orthologous regions of rice, Brachypodium, and sorghum, At3DS contains 38.67% more genes. In comparison to At3DS, the short arm sequence of wheat chromosome 3B (Ta3BS) is 95-Mb large in size, which is primarily due to the expansion of the non-centromeric region, suggesting that transposable element (TE) bursts in Ta3B likely occurred there. Also, the size increase is accompanied by a proportional increase in gene number in Ta3BS. We found that in the sequence of short arm of wheat chromosome 3D (Ta3DS), there was only less than 0.27% gene loss compared to At3DS. Our study reveals divergent evolution of grass genomes and provides new insights into sequence changes in the polyploid wheat genome.

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期刊信息
  • 《遗传学报:英文版》
  • 北大核心期刊(2004版)
  • 主管单位:中国科学院
  • 主办单位:中国科学院遗传与发育生物学研究所 中国遗传学会
  • 主编:薛勇彪
  • 地址:北京市安定门外大屯路中科院遗传发育所
  • 邮编:100101
  • 邮箱:ycxb@genetics.ac.cn
  • 电话:010-64807669
  • 国际标准刊号:ISSN:1673-8527
  • 国内统一刊号:ISSN:11-5450/R
  • 邮发代号:2-819
  • 获奖情况:
  • 1996年获中科院优秀期刊二等奖,1997年获全国优秀期刊三等奖,200年获中科院优秀期刊二等奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,荷兰文摘与引文数据库,荷兰医学文摘,美国生物医学检索系统,美国科学引文索引(扩展库),美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2000版)
  • 被引量:17519