中文摘要：

H5N1 鸟的流行性感冒病毒(AIV ) 广泛地在亚洲，欧洲和非洲传播了，做大量经济损失。最近，我们的研究组屏蔽了普通抵销单音的同种细胞的抗体把 8H5 称为，它能抵销几乎所有 H5 子类型到目前为止曾经孤立的 AIV。显然，这单音的同种细胞的抗体将处于高变化率对 H5N1 AIV 为通用 AIV 疫苗的研究和这药的设计受益。在这研究，当模特儿的相同被使用产生 8H5 Fab 碎片，和“正规结构”的 3D 结构方法被用来定义 CDR 区域的指定的环符合构造。模型在卓见 II 节目与发现模块在 cvff 力量地里受到精力最小化。产生模型有从 Ramachandran 阴谋计算和由相互作用精力分析估计了的好 3D 结构相容性计量了的正确立体化学，溶剂可存取的表面(SAS ) 分析，和 Profiles-3D 来临。而且， 8H5 Fab 模型受到与三 H5 子类型红血球凝聚素停靠(哈) 在 PDB 扔的结构(标志没有：1jsm， 2ibx 和 2fk0 ) 分别地。结果显示三停靠建筑群分享一个普通有约束力的接口，但是在联系与的有约束力的角度不同哈病毒的子类型的结构类似。根据三哈结构的相同分析的接口，普通抵销 epitope 在上哈由 8H5 认出了由 9 不连续的氨基酸残余组成：毒蛇(68 ) ， Asn (72 ) ， Glu (112 ) ， Lys (113 ) ， Ile (114 ) ，专业版(118 ) ，重量的单位(120 ) ， Tyr (137 ) ， Tyr (252 )( 至于 1jsm 数了顺序) 。现在的工作的主要目的是提供某卓见进结构和 H5N1 AIV 的普通抵销 epitope 的有约束力的细节，从而帮助通用 AIV 疫苗和抗病毒的基于结构的设计治疗学的药。

英文摘要：

The H5N1 avian influenza virus （AIV） has widely spread in Asia, Europe and Africa, making a large amount of economic loss. Recently, our research group has screened a common neutralizing mono-clonal antibody named 8H5, which can neutralize almost all H5 subtype AIV ever isolated so far. Obviously, this monoclonal antibody would benefit for research and development of the universal AIV vac-cine and design of the drug against H5N1 AIV in high mutation rate. In this study, the homology modeling was applied to generate the 3D structure of 8H5 Fab fragment, and ＂canonical structure＂ method was used to define the specified loop conformation of CDR regions. The model was subjected to energy minimization in cvff force field with Discovery module in Insight II program. The resulting model has correct stereochemistry as gauged from the Ramachandran plot calculation and good 3D-structure compatibility as assessed by interaction energy analysis, solvent accessible surface （SAS） analysis, and Profiles-3D approach. Furthermore, the 8H5 Fab model was subjected to docking with three H5 subtype hemagglutinin （HA） structures deposited in PDB （ID No： ljsm, 2ibx and 2fk0） respectively. The result indicates that the three docked complexes share a common binding interface, but differ in binding angle related with HA structure similarity between viral subtypes. In the light of the three HA inter-faces with structural homology analysis, the common neutralizing epitope on HA recognized by 8H5 consists of 9 incontinuous amino acid residues： Asp^58, Asn^72, Glu^112, Lys^113, lie^114, Pro^118, Ser^120, Tyr^137, Tyr^252 （numbered as for ljsm sequence）. The primary purpose of the present work is to provide some insight into structure and binding details of a common neutralizing epitope of H5N1 AIV, thereby aiding in the structure-based design of universal AIV vaccines and anti-virus therapeutic drugs.