中文摘要：

目的 探讨HIV Tat蛋白是否可以在细胞内影响主要节律基因Clock，Cry，Bmal1，Per的表达。方法 用lipofectamineTM2000将HIVat表达质粒转入PC12细胞，使Tat在PC12细胞中表达。转染48h后提取mRNA和蛋白，分别通过实时荧光定量PCR技术和Westernblot技术检测节律基因在mRNA和蛋白水平表达的变化。结果 研究结果显示，与未处理组和空质粒组相比，转染组Cry1的表达水平显著升高（P＜0.05）；Clock的表达水平显著降低（P＜0.05）；Bmal1和Per的表达水平无明显变化（P＞0.05）。 结论 HIV Tat影响了近日节律基因Cry1和Clock的表达，表现为Cry1表达水平升高，Clock表达水平下降，提示TAT蛋白可以影响节律基因的表达并且可能通过对近日节律的影响调节细胞生理功能。

英文摘要：

Objective To explore whether HIV TATprotein can affect the expression of Clock, Cry,Bmal1,Per in the cell. Methods HIVTat protein expression plasmids were transfected into PC12 with lipofectamine TM 2000, and Tat was expressed in PC12.mRNA and protein were extracted 48 hours after transfection, and the expression of rhythm gene was detected by realtime quantitative PCR and Western blot, respectively.Results The results showed that the expression level of Cry1was significantly increased（P〈0.05）. The expression ofClock was significantly decreased（P〈0.05）. The expression of Bmal1and Perwas not significantly changed in transfected Tatexpressing plasmid compared with untransfected and empty plasmid. Conclusion HIV Tat affects the expression of the circadian genes Cry1 and Clock, which is related to the decrease ofClock expression and the increase of Cry1 expression, Suggesting that TAT protein can affect the expression of rhythmic genes and may regulate the physiological function of cells through the influence of circadian rhythm.