中文摘要：

背景以往对近视眼巩膜力学特性变化的研究局限于其巩膜整体和条带的力学特性变化方面，目前对近视眼细胞水平力学特性的研究日益受到重视。目的明确TGF-β2对豚鼠镜片诱导型近视眼后极部巩膜成纤维细胞力学特性的影响。方法2周龄豚鼠12只，随机选取一侧眼用镜片诱导方法制备近视动物模型，对侧眼为对照。造模30d后，用组织块培养法培养豚鼠后极部巩膜成纤维细胞并传2代，采用免疫组织化学法以兔抗鼠波形蛋白、结蛋白、角蛋白、S-100抗体进行细胞鉴定。将不同质量浓度的TGF-β2（分别为0、1、10、100mg／L）加入无血清DMEM中作用24h，利用细胞微管吸吮的方法测定各组巩膜成纤维细胞的力学特性。结果模型眼0mg／LTGF—p：组与对照眼0mg／LTGF—p：组比较，模型眼的巩膜成纤维细胞的平衡杨氏模量、黏弹性参数明显升高，二者之间差异有统计学意义（P〈0．05）。模型眼与对照眼在TGF-β2作用下，0mg／L组与1mg／L组、10mg／组比较，细胞的平衡杨氏模量、黏弹性参数差异均有统计学意义（P〈0．05）。模型眼和对照眼培养细胞的平衡杨氏模量、黏弹性参数与TGF-β2的质量浓度均呈正相关（r=0．743、r=0．533；r=0．654、r=0．576），模型眼和对照眼中的0mg／L组与100mg／L组比较细胞的平衡杨氏模量、黏弹性参数差异均无统计学意义（P〉0．05），模型眼1mg／L组、10mg／L组与对照眼1mg／L组、10mg／L组比较，巩膜成纤维细胞的平衡杨氏模量、黏弹性参数之间差异有统计学意义（P〈0．05）；模型眼组100mg／LTGF-β2与对照眼的100mg／LTGF-β2组比较，巩膜成纤维细胞的平衡杨氏模量、黏弹性参数差异无统计学意义（P〉0．05）。结论随着TGF—B，质量浓度的增加，近视眼巩膜成纤维细胞的平衡杨氏模量、黏弹性参数值明显升高，1mg／L和10mg／LTGF-β2可以降低正

英文摘要：

Background There have been an abundance of literature on the analysis of the mechanical characteristic of the sclera at the entire seleral level in high myopia. However, some recent studies on high myopia are focused on the mechanical changes of the sclera on a cellular level. Objective This experiment was purposed to study how transforming growth factor-β2 （ TGF-β2 ） affected sclerotic desmocytes and the mechanical behaviors ok＂ seleral fibroblasts in the posterior part of the eyes in guinea pigs with experimental myopia. Methods Induced myopic animal models were established by wearing -10. 00 D concave lens for 30 clays in lateral eyes of 2-week-old guinea pigs. The fellow eyes were used as control group. Another 5 matched animals served as normal controls. The seleral fibroblasts of each group were purified with the tissue explant method and passaged for 2 generations in vitro.Cultured cells were identified by immunochemistly with vimentin, desmin, keratin and S-100 antibodies. Different concentrations of TGF-β2（0, 1 , 10,100 rag/L） were added into serum-free DMEM for 24 hours, and the viscoelastic properties of scleral fibroblasts were measured by micropipette aspiration technique. Results Compared with the fellow eyes and normal control eyes, the equilibrium modulus and apparent viscosity in model eyes were significantly higher （ P〈0.05 ）. After treatment of TGF-β2 , the equilibrium modulus and apparent viscosity in the model group and fellow eyes were positively correlated with the concentrations of TGF-β2 （ r = 0. 743, r = 0. 533, r = O. 654, r = 0. 576, P〈 0.05）. Following the addition of i mg/L TGF-I32 and 10 mg/L TGF-β2, the equilibrium modulus and apparent viscosity of scleral fibroblasts were significantly reduced in model eyes compared with fellow eyes （P〈 O. 05 ）. No significant difference was found in the equilibrium modulus and apparent viscosity of scleral fibroblasts between model eyes and fellow eyes after treatment with 100 mg/L TGF-β2 （P〉0. 05 ）. Concl