中文摘要：

从培植前胚囊阶段胚胎导出的胚胎的茎(ES ) 房间广泛地被用来调查决定 pluripotency 和房间系区别的分子的事件。作为首先发现的 ES 特定的抄写因素， Oct4 被看作了 ES 房间的核心 pluripotency 因素。在现在的学习，我们成功地从从女 OG2 收集的胚囊建立了七根 ES 线(Oct4-GFP 转基因) 老鼠，它与男 rtTA 被穿过转基因的老鼠。ES 房间线的 pluripotency 能被 Oct4-GFP 的表示设想在下面荧光灯显微镜学和细菌线传播能力进一步被证实了。更重要地， rtTA 的存在能在 doxycycline 的帮助下导致 transgenes 表示。因此，这些 ES 房间线提供一个优秀工具进一步发现影响 pluripotency 的新奇因素并且在定义抄写因素调查 reprogramming 的分子的机制调停的原子 reprogramming。

英文摘要：

Embryonic stem （ES） cells derived from the pre-implantation blastocyst-stage embryos have been widely used to investigate the molecular events determining pluripotency and cell lineage differentiation. As the first discovered ES-specific transcription factor, Oct4 has been considered as the core pluripotency factor of ES cells. In the present study, we successfully established seven ES lines from the blastocysts collected from female OG2 （Oct4-GFP transgenic） mice, which have been crossed with male rtTA transgenic mice. The pluripotency of the ES cell lines can be visualized by the expression of Oct4-GFP under fluorescent microscopy and germ-line transmission capability has been further confirmed. More importantly, the presence of rtTA could induce transgene＇s expression with the help of doxycycline. Therefore, these ES cell lines provide an excellent tool to further discover novel factors affecting pluripotency and to investigate the molecular mechanism of reprogramming in defined transcription factors mediated nuclear reprogramming.