中文摘要：

目的考察灯盏乙素结构修饰物灯盏乙素乙酯的透血脑屏障性能，对脑神经细胞的保护作用，并探讨其作用机制。方法采用体外血脑屏障模型，结合HPLC技术评价药物的透血脑屏障能力，采用体外原代培养大鼠大脑皮层神经细胞。结合噻唑蓝法测定细胞存活率，采用激光共聚焦技术评价药物对谷氨酸、氯化钾诱导细胞内钙超载的影响。结果与对照组比较，灯盏乙素乙酯对血脑屏障的透过率可达86．9％，能明显提高细胞存活率（P〈0．01），且对谷氨酸致神经细胞内钙超载有拮抗作用（P〈0．01）。结论灯盏乙素乙酯能有效穿透血脑屏障，且能通过对抗细胞内钙离子超载发挥脑缺血神经保护作用。

英文摘要：

OBJECTIVE To observe the blood-brain barrier permeability of scutellarin ethyl ester, the structural modification product of scutellarin, and to explore the mechanism of nerve protection on cerebral ischemia. METHODS By co-culturing brain mi- crovascular endothelial cells （BMVEC） and astrocytes cells （AC） in vitro, blood-brain barrier model was established. The active com- ponents were analyzed by HPLC. Rat cerebral cortical neurons cells were cultivated in vitro, and the survival rate was determined by MTT assay. The model of rat cortical neurons cell injury was established by glutamate and KCl, and the change of fluorescence intensity was detected by Laser Scanning Confocal Microscopy （LSCM）. RESULTS Compared with control group, scutellarin ethyl ester could penetrate BBB, promote the survival of cortex nerve cells in vitro significantly（ P 〈 0.01 ）, and inhibit intracellular calcium overload in- duced by 500 μmol·L^-1 glutamate （P 〈 0. 01 ）. CONCLUSION Scutellarin ethyl ester can pass through BBB and has neuroprotec- tive effect by regulation of calcium signal pathway to resist cerebral ischemia.