以陆地棉早熟品种中棉所58和中熟品种邯郸109为材料,研究子叶衰老过程中氮素的再利用率(NRE)及氮代谢相关指标的变化。结果表明:子叶衰老过程中,单位面积总N含量(NPA)逐渐降低,中棉所58 NRE为69.26%,邯郸109为54.45%;叶绿素、可溶性蛋白质含量和硝酸还原酶(NR)活性先上升后降低,NR最先降低,中棉所58和邯郸109分别在9d、14d开始下降;中棉所58各指标比邯郸109早下降4~8d。谷氨酸脱氢酶(GDH)活性先下降后上升,表明GDH可能是氮素再利用的关键酶。
Nitrogen (N) resorption efficiency (NRE) and changes in nitrogen metabolism indices in cotton cotyledons during leaf senescence were studied in two cotton cultivars: CCRI 58 (short season) and Handan 109 (medium season). The main results were as follows: during the process of leaf growth and senescence, total nitrogen content in unit area (NPA) decreased gradually, NRE in CCRI 58 and Handan 109 is separately 69.26% and 54.45%, N metabolism indices such as chlorophyll and soluble protein content and nitrate reductase (NR) activity, are all increased first, then declined. The first to show a decline was NR activity, 9 d and 14 d in CCRI 58 and Handan 109. The declined times in CCRI 58 are 4-8 days prior to Han 109. Glutamate dehydrogenase (GDH) activity first declined, then increased, indicting that GDH may be the key enzyme involved in N resorption.