中文摘要：

目的建立DBA/1J小鼠胶原诱导关节炎（CIA小鼠）模型,初步研究CIA小鼠外周血单个核细胞中及病变关节组织中BMPs的表达水平。方法利用牛Ⅱ型胶原诱导DBA/1J小鼠发生关节炎病理改变;利用荧光定量PCR检测发病外周血单个核细胞中及关节组织BMPs的mRNA的表达水平,利用免疫荧光技术检测关节组织中BMP9蛋白表达水平。结果成功建立了CIA小鼠模型,检测发现BMP4mRNA和BMP9mRNA在发病的CIA小鼠外周血单个核细胞及病变关节组织中表达明显下调（P〈0.05）,荧光免疫技术显示BMP9蛋白在CIA小鼠病变关节组织中表达明显下调（P=0.002）。结论在基因水平BMP4、9在CIA小鼠外周血单个核细胞中及病变组织中表达明显下调,蛋白水平BMP9在CIA小鼠病变组织中表达明显下调。

英文摘要：

Objective To establish collagen-induced arthritis （CIA） model used of DBA/1J mouse,a preliminary study of the expression levels of BMPs in mononuclear cells of peripheral blood and the joints tissue of CIA mice. Methods We induced DBA/1J mice and developed arthritis pathology by using of bovine type Ⅱ;then, we detected rnRNA and protein expression levels of BMPs by using quantitative PCR and immunohistochemical staining. Results We successfully established CIA mouse model. The expres- sion of BMP4 and BMP9 mRNA was significantly down-regulated in peripheral blood mononuclear cells and in the pathogenesis of joint tissues of CIA mice （P〈0.05）. It showed that BMP9 protein significantly decreased in joint tissues of CIA mice by immuno- fluorescence technique （P= 0. 002）. Conclusion At the genetic level, the expression of BMP4 and BMP9 could be significantly down-regulated in the CIA mouse. At the protein level,BMP9 could be significantly down-regulated in the CIA mouse.