中文摘要：

目的评价吸入氢气对脓毒症小鼠急性肺损伤时炎性反应的影响。方法雄性ICR小鼠48只，体重20～25g，5周龄，采用随机数字表法，将其随机分为4组（n=12）：假手术组（A组）、假手术＋氢气组（B组）、脓毒症组（c组）和脓毒症＋氢气组（D组）。采用盲肠结扎穿孔（cLP）法制备脓毒症模型。B组和D组于假手术或CLP后1、6h吸入2％氢气1h。CLP后24h时行行为学评分，随后采集动脉血行血气分析，计算氧合指数；采集静脉血样，随后处死小鼠取肺组织，测定血清及肺组织TNF—α、IL-1β、高迁移率族蛋白B1（HMGB1）和IL-10水平和计算凋亡指数。结果与A组比较，C组和D组行为学评分升高，氧合指数降低，凋亡指数升高，血清及肺组织TNF-α、IL-1β、HMGB1和IL-10水平升高（P〈0．05），B组差异无统计学意义（P〉0．05）；与C组比较，D组行为学评分降低，氧合指数升高，凋亡指数降低，血清及肺组织TNF-α、IL-1β和HMGB1水平降低，IL-10水平升高（P〈0．05）。结论吸人氢气可调节促炎和抗炎因子水平平衡，抑制炎性反应，减轻脓毒症小鼠急性肺损伤。

英文摘要：

Objective To investigate the effect of inhalation of hydrogen （H2 ） on the inflammation re- sponse during sepsis-induced acute lung injury in mice. Methods Forty-eight male ICR mice, aged 5 weeks, weighing 20-25 g, were randomly divided into 4 groups （ n = 12 each） ： sham operation group （group A）, sham operation ＋ H2 group （group B）, sepsis group （group C） and sepsis ＋ H2 group （group D）. Sepsis was pro- duced by cecal ligation and puncture （CLP） . Groups B and D received 1 h inhalation of 2% H2 at 1 and 6 h after CLP or sham operation. The behavior was assessed and scored at 24 h after CLP. Arterial blood samples were tak- en for blood gas analysis. Oxygenation index was calculated. Venous blood samples were taken for determination of the levels of serum tumor necrosis factor-α （TNF-α）, interleukin-I （IL-1β）, high mobility group protein B1 （HMGB1） and interleukin-10 （IL-10）. The mice were then sacrificed and lungs were removed for determination of the levels of TNF-a, IL-1β, HMGB1 and IL-10 in lung tissues. The apoptotic index was calculated. Results Compared with group A, the behavior score, apoptotic index and levels of TNF-a, IL-1β, HMGB1 and IL-10 were increased, and the oxygenation index was decreased in groups C and D, and no significant change was found in the parameters mentioned above in group B. Compare with group C, the oxygenation index and level of IL-10 were sig- nificantly increased, and the behavior score, apoptotic index and levels of TNF-α, IL-1β and HMGBI were signifi- cantly decreased in group D. Conclusion Inhalation of H2 can regulate the balance between pro- and anti-inflam- matory eytokines, inhibit the inflammation response and alleviate sepsis-induced acute lung injury in mice.