中文摘要：

目的研究白细胞介素13（IL-13）对红白血病细胞系HEL细胞分化作用及对HEL细胞转录因子c-fos表达的影响。方法用RT—PCR方法检测HEL细胞IL-13受体仪1、GPⅡb、vWF和c-fos基因mRNA表达，用Western blot和流式细胞术分别检测IL-13受体α1、c-fos、GPⅡb和vwF蛋白水平的表达。结果HEL细胞表达IL-13受体α1，用IL-13（100ng／ml）作用于HEL细胞，GPⅡb和vWF基因mRNA表达上调，实验组和对照组GPⅡb／β-actin吸光度比值分别为2．912，1．303，实验组为对照组的2．23倍（P〈0．05）；实验组和对照组vWV／f3一actin吸光度比值分别为0．506，0．217，实验组为对照组的2．33倍（P〈0．05）。流式细胞术分析结果显示，实验组GPⅡb和vWF蛋白表达明显高于对照组。IL-13作用于HEL细胞30min，c—fos基因mRNA表达水平达高峰，IL-13作用HEL细胞60min，c—fos蛋白表达达高峰，30min组、60min组c-fos/β-actin吸光度比值高于其它各组（P〈0．05）。结论IL-13可促进HEL细胞分化，并上调c-fos表达。

英文摘要：

Objective To study the effects of IL-13 on the differentiation and expression of transcription factor c-los of human erythroleukemia cell line （HEL） cells. Methods Reverse transcription polymerase chain reaction （RT-PCR） was used to observe the mRNA expression of IL-13 receptor α1, GP Ⅱ b, vWF and c-los, and Western blot and cytometry were used to analyse their protein expression. Results IL-13 receptor α1 was expressed on HEL cells. IL-13 （ 100 ng/ml ） up-regulated the mRNA expression of GP Ⅱ b and vWF. The ratio of luminous absorption （ LA ） of GPⅡb to β-actin bands （ AB ） was 1. 303 in control group, whereas was 2. 912 in experiment group; being 2.23-fold higher than that in control group （P〈0.05）. The ratio of LA to AB for vWF was 0.217 in control group, and 0.506 in experiment group; indicating a 2.33-fold increase in experiment group （ P〈0.05 ）. The protein expression of GP Ⅱb and vWF was significantly increased in experiment group, compared with that in control group. IL-13 inducing the increased expression of c-los mRNA and protein of HEL cells peaked at 30 rain and 60 rain, respectively. The ratio of LA to AB for c-fos was also increased at 30 rain and 60 rain （ P 〈 0.05 ）. Conclusion IL-13 prompts the differentiation of HEL cells and up-regulates the expression of c-los.