中文摘要：

目的研究海风藤提取物对纤丝状和寡聚体p淀粉样蛋白（B—amyloid，Ap）诱导的活化新生大鼠小胶质细胞可能的影响。方法原代培养新生大鼠小胶质细胞，分别用纤丝状A[325—35、寡聚体A~25-35、纤丝状A[325-35＋海风藤提取物、寡聚体A[325-35＋海风藤提取物干预小胶质细胞，并设立空白对照组。干预48h后，以CD68作为小胶质细胞活化的特异性抗原标志，利用免疫细胞化学染色方法，对小胶质细胞的活化率进行检测，并比较不同处理组间的差异。结果四个实验组的小胶质细胞CD68表达阳性率均高于空白对照组（5．1％，P〈0．05）。纤丝状AB＋海风藤提取物组CD68表达阳性率（52．1％）低于纤丝状AB组（60．8％，P〈0．05）。寡聚体A[3组（71．2％）和寡聚体A[3＋海风藤提取物组（67．0％）的CD68表达阳性率无明显差异（P=0．101）。结论纤丝状和寡聚体AB均能激活小胶质细胞；海风藤提取物能够抑制纤丝状Ap对小胶质细胞的激活作用，对寡聚体Ap的激活作用无明显影响。

英文摘要：

Objective To compare the activation of microglia in vitro induced by oligomeric and fibrillar Aβ, and research the effects of Piper Kadsura Ohwi extracts on the microglial activation. Methods Microglia were divided randomly into 4 test groups,intervened by fibrillar Aβ25-35, fibrillar Aβ25-35 ＋ Piper Kadsura Ohwi extracts,oligomeric Aβ25-35 and oligomefic Aβ25-35 ＋ Piper Kadsura Ohwi extracts respectively. Also a blank contrast group was set without any intervention. 48 hours later,activation of microglia was tested by immunocytochemistry, using the CD68 molecule as a specific marker of microglial activation and the incidences of active microglia in different groups were compared. Results Each of the 4 test groups had a higher positive incidence of CD68 expression than that of the blank contrast （ 5.1% ） （P 〈 0.05 ） ; positive incidence of fibrillar Aβ ＋ Piper Kadsura Ohwi extracts group （52.1% ） was lower than that of the fibrillar Aβ group （ 60.8 % ） （P 〈 0.05 ） ; positive incidence of oligomeric Aβ ＋ Piper Kadsura Ohwi extracts group （67.0%） was not significantly different with that of the oligomeric Aβ group （71.2%）, P=0. 101. Conclusion Both fibrillar and oligomeric Aβ has the ability to activate the silent microglia. Piper Kadsura Ohwi extracts could inhibit the activation of microglia induced by fibrillar Aβ25-35 ,but didnt show significant effects on the activation induced by oligomeric Aβ25-35.