中文摘要：

背景 NR2B 包含 N-methyl-D-aspartate (NMDA ) 受体在神经病的疼痛的容易和维护起一个重要作用。在中央神经系统(CNS ) 的 NR2B 子单元的分离分发可以支持在这个地点有选择地行动的代理人的减少的副作用。因此，我们调查了指向 NMDA 受体的 NR2B 子单元的体液的自体免疫的回答减轻的假设象外部 injury.Methods 老鼠生产的行为一样的疼痛在二星期的间隔与 NR2B 锁眼恋栈者 Hemocyanin (NR2B-KLH ) 皮下地被使免疫三次。在 sera 和脑髓的液体的 NR2B 特定的 IgG titres 被间接 ELISA 决定。在第三免疫以后的七天，臀部的神经(胫骨、普通的小腿侧的神经) 的 3 个终端分支中的 2 个紧被绑扎。行为的测试在外科以后在每隔一天被执行，直到在外科以后的 7 天。腰部的针的绳索(L4-6 ) 在在结扎以后的白天 7 上被移开。在腰部的针的绳索的 NR2B 蛋白质的表示在第二种痘以后用西方的 blotting.Results 被决定，在 sera 的 NR2B 特定的 IgG 被检测是在九只老鼠中的六个的 0.5 g/ml。在第三种痘以后，所有使免疫的老鼠有 2.2 g/ml。在 sera 的 NR2B 特定的 IgG 的 Titres 达到顶点 42 天张贴起始的免疫并且坚持了超过 70 天。没有 NR2B 特定的 IgG 从 PBS 或 KLH group.The 在 sera 被检测行为在 NR2B 组的阀值比在在结扎以后的白天 7 上的 PBS 和 KLH 组的那些显著地高。在在 NR2B 组的 CSF 的 NR2B 特定的 IgG 不能在在针的解剖前的白天 1 上被检测；但是能在在外科以后的白天 7 上被检测。在组 NR2B 的 NR2B 蛋白质的表示比在在 surgery.Conclusion 以后的白天 7 上的 PBS 和 KLH 组 NR2B 肽能对神经病的疼痛被用作一支疫苗的显著地低，它能在腰部的针的绳索与 NR2B 蛋白质的减小被联系。

英文摘要：

Background NR2B containing N-methyI-D-aspartate （NMDA） receptor plays an important role in the facilitation and maintenance of neuropathic pain. The discrete distribution of NR2B subunit in the central nervous system （CNS） may support reduced side effects of agents that act selectively at this site. Therefore, we investigated the hypothesis that a humoral autoimmune response targeting the NR2B subunit of NMDA receptor relieves pain like behaviours produced by peripheral injury. Methods Rats were immunized subcutaneously with NR2B-Keyhole Limpet Hemocyanin （NR2B-KLH） three times at two-week intervals. NR2B specific IgG titres in sera and cerebrospinal fluid were determined by indirect ELISA. Seven days after the third immunization, 2 of the 3 terminal branches of the sciatic nerve （tibial and common peroneal nerves） were tightly ligated. Behavioural testing was carried out on every other day after surgery, until 7 days after surgery. The lumbar spinal cord （L4-6） was removed on day 7 after ligation. The expression of NR2B protein in the lumbar spinal cord was determined using Western blotting. Results After the second vaccination, NR2B specific IgG in sera was detected to be 〉0.5 μg/ml in six of nine rats. After the third vaccination, all the immunized rats had 〉2.2 μg/ml. Titres of NR2B specific IgG in sera peaked 42 days post initial immunization and persisted for over 70 days. No NR2B specific IgG was detected in sera from PBS or KLH group. The behavioural thresholds in NR2B group were significantly higher than those in PBS and KLH groups on day 7 after ligation. NR2B specific IgG in CSF in NR2B group could not be detected on day 1 before spinal dissection; but could be detected on day 7 after surgery. The expression of NR2B protein in group NR2B was significantly lower than in PBS and KLH groups on day 7 after surgery. Conclusion The NR2B peptide could be used as a vaccine against neuropathic pain, which could be associated with reduction of NR2B protein in the lumbar spinal cor