中文摘要：

它是众所周知的那 Tn5B1-4 (商业地作为高度知道五) 房间线高度产生 baculovirus 并且当与另外的昆虫相比房间排队时，提供 recombinant 蛋白质的优异生产。但是房间线的特征总是不仍然保持稳定并且可以在连续经过之上变化。最近 alphanodavirus，命名 Tn5 房间线病毒(或 TNCL 病毒) ，特别地在高度五房间被识别。因此，我们建立了一根新房间线， QB-Tn9-4s 从 Trichoplusia ni，它决心没有由 RT-PCR 分析的 TNCL 病毒。在这份报纸，我们描述新奇房间克隆的发展， QB-CL-B，从一个低经过 QB-Tn9-4s 房间衬里并且报导它的危险性到 recombinant 的 AcMNPV，和水平蛋白质生产。这细胞克隆类似于它在形态学和生长率的父母细胞 QB-Tn9-4s 和 Tn5B1-4 细胞；尽管它也近似显示出一样的回答到 AcMNPV 感染和吸藏身体的生产，有与 QB-Tn9-4s (父母房间) 和 High5 房间相比的 recombinant 蛋白质生产的高水平。

英文摘要：

It is well known that Tn5B1-4 （commercially known as the High Five） cell line is highly susceptible to baculovirus and provides superior production of recombinant proteins when compared to other insect cell lines. But the characteristics of the cell line do not always remain stable and may change upon continuous passage. Recently an alphanodavirus, named Tn5 Cell Line Virus （or TNCL Virus）, was identified in High Five cells in particular. Therefore, we established a new cell line, QB-Tn9-4s, from Trichoplusia ni, which was determined to be free of TNCL virus by RT-PCR analysis. In this paper, we describe the development of a novel cell clone, QB-CL-B, from a low passage QB-Tn9-4s cell line and report its susceptibility to AcMNPV, and the level of recombinant protein production. This cell clone was similar to its parental cells QB-Tn9-4s and Tn5B1-4 cells in morphology and growth rate; although it also showed approximately the same responses to AcMNPV infection and production of occlusion bodies, there were higher levels of recombinant protein production in comparison to QB-Tn9-4s （parental cells） and High5 cells.