中文摘要：

为明确云南红壤区紫花苜蓿（Medicago sativa）亲本及其杂种后代的遗传变异,采用了不连续聚丙烯酰胺凝胶电泳技术,对分别以‘德钦’苜蓿与‘Acrora’为父母亲本的正反杂交组合的亲本、F1代和F2代过氧化物同工酶进行分析.结果表明：‘德钦’苜蓿♀与‘Acrora’♂杂交的同工酶谱带有10条,F2代之间的遗传距离最大为0.60;‘德钦’苜蓿♂与‘Acrora’罕杂交的同工酶谱带有8条,F2代之间的遗传距离最大为0.50.以‘德钦,苜蓿为母本F2代之间的变异比以‘德钦’苜蓿为父本F2代之间的变异大.不同遗传背景对紫花苜蓿后代POD同工酶酶谱特征的影响较大.

英文摘要：

Peroxidase （POD） is ozymes of parent and hybrids of reciprocal cross combination were analyzed by PAGE in order to study their genetic variation in red soil in Yunnan province. The result showed that the 10 enzyme bands of POD isozymes were separated from ‘Deqin＇ ♀ and ‘Acrora＇ ♂as parent and their F1, F2hybrids. The largest genetic distance was 0. 60 among F2 hybrids. The 8 enzyme bands of POD isozymes were separated from ‘Deqin＇ ♂ and ‘Acrora＇ ♀ as parent and their F1 , F2 hybrids. The largest genetic distance was 0.50 among F2 hybrids. The variation among the F2 hybrids of Deqin♀ as parent was bigger than that of‘Deqin’ ♂ and ‘Acror’♀ as parent. So the peroxidase isozyme features of alfalfa hy- brids were influenced by different genetic backgrounds.