中文摘要：

目的观察牛蒡低聚果糖（BFOS）对RAW264.7炎症细胞模型的抗炎作用。方法用脂多糖（LPS）处理巨噬细胞系RAW264.7细胞,建立炎症模型,并用不同浓度BFOS处理。采用定量PCR检测环氧合酶2（COX-2）、一氧化氮合成酶（iNOS）、单核细胞趋化蛋白1（MCP-1）、白细胞介素6（IL-6）等炎性因子的表达水平,ELISA检测BFOS处理前后MCP-1、IL-6的分泌量。Western blot法检测COX-2、iNOS、κB抑制因子（IκB）、核转录因子κB p65（NF-κBp65）、细胞外信号调节激酶（Erk）、P38、c-Jun氨基末端激酶（JNK）的磷酸化蛋白表达水平。结果与对照组相比,经BFOS处理后,细胞iNOS、COX-2、MCP-1及IL-6的水平表达显著下降（P〈0.01）。BFOS处理组p-IκB、p-NF-κBp65、p-Erk、p-P38、p-JNK等蛋白表达较LPS单独处理组显著下降（P〈0.01）。结论 BFOS对LPS诱导的RAW264.7细胞炎症模型具有抗炎作用。

英文摘要：

Objective To investigate the anti-inflammatory effect of burdock fructo-oligsaccharide （BFOS） on the in- flammatory model of RAW264.7 cells. Methods The inflammation model was constructed by treating RAW264.7 cells with lipopolysaccharide （ LPS ）, and treated with different doses of BFOS. Expressions of Cyclooxygenase （ COX- 2）, Nitric oxide synthases （ iNOS）, monocyte chemotactic protein-1 （ MCP-1 ） and Interleukin-6 （IL-6） were detected by real-time PCR. Expressions of MCP-1 and IL-6 were determined by ELISA before and after the treatment with BFOS. Expressions of COX-2, iNOS, phosphorylated IκB, NF-κBp65, extracellular signal-regulated （Erk）, P38 and c-Jun N-terminal kinase （JNK） were detected by Western blot. Results The expression levels of iNOS, COX-2, MCP- 1 and IL-6 of RAW264.7 cells stimulated by LPS increased, and BFOS significantly decreased them. BFOS decreased the expressions of p-IκB,p-NF-κBp65,p-Erk,p-P38, and p-JNK compared to the control. Conclusion BFOS may ex- ert anti-inflammatory effect on RAW264.7 cells stimulated by LPS.