中文摘要：

目的研究积雪草苷对增生性瘢痕成纤维细胞结缔组织生长因子（CTGF）的影响及RhoA/ROCK-Ⅰ信号通路在其中的作用。方法分离培养人增生性瘢痕组织来源的成纤维细胞,应用积雪草苷300μg.mL-1及RhoA的下游效应器Rho激酶的特异抑制剂Y-27632对实验细胞进行干扰实验。分为对照组、积雪草苷组、Y-27632组、积雪草苷＋Y-27632组。运用荧光定量PCR（SYBR Green）及免疫荧光细胞化学的方法检测各组瘢痕成纤维细胞中RhA、ROCK-Ⅰ、CTGF mRNA与蛋白表达。结果积雪草苷300μg.mL-1能抑制RhoA、ROCK-Ⅰ、CTGF的mRNA与蛋白表达,与对照组比较具有统计学意义（P〈0.05）;Y-27632能阻碍积雪草苷的作用,积雪草苷（300μg.mL-1）＋Y-27632组,CTGF的mRNA与蛋白表达明显高于单纯积雪草苷组（P〈0.05）,与对照组及Y-27632组比较无显著性差异（P〉0.05）;Y-27632组与对照组比较RhoA、ROCK-Ⅰ、CTGF的mRNA与蛋白表达没有统计学意义。结论积雪草苷300μg.mL-1能抑制RhoA、ROCKⅠ-、CTGF的mRNA与蛋白表达;而且可通过RhoA/ROCKⅠ-信号通路抑制瘢痕成纤维细胞中CTGF mRNA与蛋白表达。这可能是积雪草苷治疗瘢痕的作用机制之一。

英文摘要：

OBJECTIVE To study the effects of asiaticoside on connective tissue growth factor（CTGF） and RhoA/ROCK signal pathway in fibroblasts obtained from hypertrophic scar.METHODS Fibroblasts were obtained from human hypertrophic scars and cultured in the mediums,which were divided into 4 groups： the control group,the asiaticoside group,the Y-27632 group and the asiaticoside＋Y-27632 group.Fluorescent quantitative PCR assay（FQ-PCR） and immunofluorescence cytochemistry were used to quantify the expression and secretion of RhocA,ROCK-Ⅰ and CTGF in the hypertrophic scar fibroblasts of each group.RESULTS To compared with the control group,the expression and secretion of RhocA,ROCK-Ⅰ and CTGF were significantly inhibited in asiaticoside group（P0.05）.But the effects of asiaticoside were inhibited by Y-27632 group.To compared with the asiaticoside group,the expression and secretion of ROCK-Ⅰ and CTGF in the asiaticoside＋Y-27632 group were up regulated（P0.05）.But among the asiaticoside＋Y-27632 group,the Y-27632 group and the control group,no significant difference was found.Between the Y-27632 group and the control group no difference was found neither.CONCLUSION The expression and secretion of RhocA,ROCK-Ⅰ and CTGF in hypertrophic scar fibroblasts through RhoA/ROCK-Ⅰ signal pathway were inhibited by asiaticoside,which may be one of the molecular mechanism of asiaticoside in hypertrophic scar formation.