中文摘要：

目的比较C57BL/6J和C3H／HeN两种小鼠放射性肺损伤进程的异同。方法采用20Gy^60Coγ射线照射C57BL/6J和C3H／HeN两种小鼠复制动物模型，应用天狼猩红染色、羟脯氨酸含量测定、免疫组织化学等方法检测Ⅰ、Ⅲ型胶原分布、羟脯氨酸含量、纤连蛋白（FN）、层粘连蛋白（LN）和α-平滑肌肌动蛋白（α—SMA）表达变化。结果照后1、3和6个月C57BL/6J小鼠肺组织病变经历炎症期、增生期和纤维化期，胶原沉积增多，照后1和3个月FN表达明显高于对照组（P〈0．01），照后6个月逐渐减少，LN表达在照后呈渐进性增加，α-SMA表达比C3H／HeN强；照后1、3和6个月C3H／HeN小鼠肺组织主要表现为间质性炎症改变，FN表达于照后1、3和6个月与对照组相比无明显变化，LN表达于照后1和3个月明显增强，6个月逐渐减少。结论20Gy^60Coγ射线照射成功复制易，抗放射性肺纤维化动物模型。C57BL/6J小鼠发生放射性肺纤维化，以胶原沉积增多出现早且显著为特征；C3H／HeN小鼠未发生放射性肺纤维化。

英文摘要：

Objective To compare the course of radiation pulmonary injury in C57BL/6J and C3H/HeN mice. Methods C57BL/6J and C3H/HeN mice irradiated with 20 Gy ^6o Co gamma rays were used as animal models. Sirius red staining and hydroxyproline measurement were used to detect the distribution of type Ⅰ and Ⅲ collagens and the content of pulmonary hydroxyproline. Immunohistochemistry was used to observe the changes of the expressions of fibronectin （FN） and laminin （LN） and alpha-smooth muscle actin （α-SMA） in the lung. Results The changes in the lungs in C57BL/6J mice were as follows： the pathological changes undergone interstitial pneumonia, proliferation and fibrosis ; significant increase of collagen deposition; FN increased rapidly to a significantly higher level at 1 and 3 months after irradiation than that in the control （ P 〈 0.01 ）, and then decreased gradually to a normal level at 6 month after irradiation ; LN elevated gradually after irradiation; the expressions of α-SMA were more intense than that in C3H/HeN mice. The changes in the lungs in C3H/HeN mice were as follows： the pathological changes mainly developed institial pneumonitis; no significant changes of FN expression was observed after irradiation compared to that in the control; LN increased significantly at 1 and 3 months after irradiation, and then decreased gradually. Conclusions The models of radiation pulmonary fibrosissensitive and-resistant were established by irradiation with gamma rays, C57BL/6J mice developed late radiation pulmonary fibrosis, and were characterized by the significant accumulation of collagen. C3H/HeN mice did not develop radiation pulmonary fibrosis.