中文摘要：

目的：通过对哮喘大鼠肺、大肠黏膜中CD4＋、CD8＋淋巴细胞的测定,揭示＂肺与大肠相表里＂理论的物质基础及科学内涵。方法：采用免疫组化染色方法观察两个部位CD4＋、CD8＋淋巴细胞的变化。结果：（1）哮喘组大鼠的肺、大肠中CD4＋、CD8＋的表达均明显高于正常组,有统计学差异（P〈0.05）;2）正常组大鼠肺、大肠中CD4＋、CD8＋的表达无明显差异（P〉0.05）;（3）在正常组,CD4＋在肺、肠组织中的表达呈负相关性,有统计学差异（P〈0.05）;CD8＋在肺、肠组织中的表达无相关。在哮喘组CD4＋在肺、肠组织中的表达呈负相关性,有统计学差异（P〈0.05）;CD8＋在肺、肠组织中的表达无相关性。结论：（1）发现在哮喘组大鼠的肺、大肠中CD4＋、CD8＋的表达明显增高,它们可能参与了哮喘的发病;（2）两组大鼠CD4＋在肺、肠组织中的表达均呈负相关性;（3）CD4＋、CD8＋T淋巴细胞可能是＂肺与大肠相表里＂理论之间的部分物质联系。

英文摘要：

Objective：Through the research of the CD4＋ and CD8＋ lymphocytes in lung and large intestine of asthma rats,the interior-exterior relation between lung and large intestine was revealed and the material basis of the theory and scientific content were proviede.Methods：Immunohistochemical staining was used to observe two parts of the CD4＋ and CD8＋ lymphocyte changes.Results：（1）CD4 and CD＋8 expressions in lung and large intestine of asthma group rats were significantly higher than those of the normal group,with a statistically significant difference（P0.05）.（2）CD＋4 and CD＋8 expressions in lung and large intestine of normal group rats had no significant difference（P0.05）.（3）In the normal group,CD4＋ expressions in the lung and intestinal tissues were negatively correlated,with a statistically significant difference（P0.05）;CD＋8 expressions in the lung and intestinal tissues had no correlation.CD＋4 expressions in the asthmatic lung and intestinal tissues were negatively correlated,with a statistically significant difference（P0.05）;CD＋8 expressions in the lung and intestinal tissues had no correlation.Conclusion：（1）Through the research,we find that CD＋4 and CD＋8 expressions in lung and large intestine of asthmatic rats could be significantly increased,they may be involved in asthma.（2）CD＋4 and CD＋8 T lymphocytes may be the material contact of the interior-exterior relation between lung and large intestine.