中文摘要：

目的：应用信号通路磷酸化广谱筛选抗体芯片技术探索菩人丹调控ob/ob小鼠糖尿病前期病变,减缓T2DM进程的可能信号通路及具体调变位点。方法：以肥胖、胰岛素抵抗为特征的ob/ob小鼠为糖尿病前期模型,利用信号通路磷酸化广谱筛选抗体芯片分析筛选空白组、模型组及菩人丹干预组胰腺组织的磷酸化修饰差异蛋白。结果：菩人丹干预组与模型组比较共筛选发现61个同空白组趋势一致的磷酸化修饰差异蛋白,PANTHER软件对其进行生物学功能分类,发现多参与机体的细胞过程、代谢过程、应激反应、生物调控、发育过程及免疫系统过程,DAVID软件进行Pathway通路富集,导入Cytoscape 3.2.1软件进行通路间关系网络互作分析,确认了ob/ob小鼠糖尿病前期的部分磷酸化修饰差异蛋白所涉及的关键通路。结论：菩人丹干预糖尿病前期病变,延缓T2DM病程发展,主要与胰岛素信号、mTOR信号通路、MAPK信号通路、凋亡、自噬通路及Ca~（2＋）通路等密切相关。

英文摘要：

Objective： Phospho Explorer Antibody Microarray was applied to explore the signal transduction regulation mechanism of Pu-Ren-Dan in ob/ob mice pancreas. Method： Setting the obesity and insulin resistant ob/ob mice as model.Pancreas from the blank group,the model group and Pu-Ren-Dan group were detected by Phospho Explorer Antibody Microarray. Result： Compared with model group,61 different phosphorylated proteins were found in Pu-Ren-Dan group,which the variation trend was consistent with the blank group. Through the biological function analysis,the found 61 proteins were mainly involved in cellular process, response to stimulus, metabolic process, biological regulation,developmental process and immune system process. The Pathway-Act-Network was built according to the interactions with pathways identified in the KEGG database,including insulin signaling pathway,mTOR signaling pathway,MAPK signaling pathway,apoptosis,calcium signaling pathway etc. Conclusion： This may be one of the molecular mechanisms which PuRen-Dan intervenes in Pre-diabetes to regress the disease.