中文摘要：

利用自组装技术将50nm的纳米金吸附到修饰了聚乙烯吡咯烷酮（PVP）的玻片上，制备得到牢固的SERS活性基底．扫描电子显微镜（SEM）对其表面粒子形貌表征表明：形成了有序的单层吸附的纳米金粒子膜，无明显团聚现象．结合Ramanmapping技术，以孔雀石绿这一禁用兽药为探针分子，观察了基底表面的SERS信号分布，计算得到各点信号的相对标准偏差低于10％．基底在水溶液中浸泡48h后，仍能保持较好的稳定性和高的SERS活性．结果表明：该基底制备简单，均匀性和重现性好，在水溶液中稳定性高，对孔雀石绿的检测限可达5×10^-9mol·L^-1.

英文摘要：

Through self- assembly technique, nano Au of 50 nm were adsorbed onto the polyvinylpyrrolidone （PVP） modified glass surface to achieve surface enhanced Raman scattering （ SERS）. SEM images revealed that the gold particles distributed uniformly to form the ordered monolayer with almost no evident aggregation, Malachite green, a prohibited animal drug, was used as probe molecule to investigate the SERS signal distribution by Raman mapping technique. The relatively standard deviation of the SERS responses on the substrate was lower than 10%. The substrate retained good stability and high SERS activity after being dipped in water for 48 h. The proposed substrate possessed the merits of easy preparation, uniform distribution, good reproducibility and strong stability against aqueous solution. The lowest detection limitation for malachite green was5×10^-9mol·L^-1.