以超级杂交稻协优9308(协青早B/中恢9308)的重组自交系(R IL)为材料,通过溶液培养试验检测苗期不同阶段与低氮耐性相关的数量性状基因座(QTL)。结果共检测到14个QTLs,单个QTL可解释的表型变异为7.13%1~3.03%。其中,处理后15 d检测到6个QTLs,分别位于第1、7、1、7、10和11染色体上;处理后30 d检测到8个QTLs,分别位于第3、8、3、10、3、8、10和4染色体上。处理后15 d,在第1染色体RM297-RM212区间检测到同时控制相对冠干重和相对总干重的QTL,与氮循环有关,此染色体区域可能富含关键的氮代谢基因。定位结果表明,两个时间检测出的低氮耐性QTL的差异表达与水稻不同发育阶段基因的时空表达密切相关,从而反映在低氮耐性位点的差异上。
A recombinant inbred lines(RIL)population derived from a cross of XQZB/R9308 of a super hybrid rice Xieyou9308 was used to detect the quantitative trait loci(QTLs)for N-deficiency tolerance at different seedling stages by using cultural experiment.A total of 14 QTLs were detected,the detected QTL individually accounted for 7.13%-13.03% of the phenotypic variation,among the QTLs,six QTLs on chromosomes 1,7,1,7,10 and 11,respectively were detected on 15 days after treatment,and eight QTLs on chromosomes 3,8,3,10,3,8,10 and 4,respectively were detected on 30 days after treatment.One QTL within the interval RM297-RM212 on chromosome 1 was detected for relative shoot dry weight and relative total dry weight,which was associated with N recycling in rice.This chromosome region may be enriched with the key N metabolism genes.The results showed that the QTL of differential expression of the two periods of N-deficiency tolerance were closely related to the genes of temporal and spatial expression of different developmental stages in rice,which reflected on the site differences of N-deficiency tolerance.