欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
本研究以循环肿瘤细胞（乳腺肿瘤细胞）为研究对象，以聚二甲基硅氧烷（PDMS）、双面粘性薄膜（DSA）、玻片为原材料，采用激光雕刻技术制作微流控芯片，结合巯基一马来酰亚胺基团硅烷化偶联法和免疫荧光技术进行芯片内捕获检测实验，并使用外周血肿瘤细胞来验证此微流控芯片的实用性，使用具有高速摄像功能的荧光显微镜进行镜下观察及拍摄．成功构建了一种简易型微流控芯片系统，利用此系统可实现对乳腺肿瘤细胞（92±3）％的捕获率，对外周血肿瘤细胞（88±3）％的捕获率，而且芯片的制作工艺简单，对实验仪器要求低，1min内即可制作完成，简化了制作过程，弥补了传统光刻工艺复杂繁琐的不足，为临床检测疾病的发生与发展提供了新的研究方向．
英文摘要：
Due to their portability, affordability and high sensitivity, microfluidic systems have become promising technolo- gies to develop point-of-care （POC） diagnostics. A major challenge in microfluidic system fabrication is to integrate multiple microfluidic components in a simple way and construct microfluidic systems in a rapid, inexpensive manner. Here we inves- tigated the design and fabrication of rapid capture of circulating tumor cells （CTCs） without the need for a cleanroom facility. The device contains is composed of a polydimethylsiloxane （PDMS） layer and a.double-sided adhesive film （DSA）. The microfluidic channels on the DSA film were simply fabricated using a laser prototyping techniquel PDMS was chosen as the support layer to facilitate sample injection and avoid leakage. The microfluidic chip fabrication takes less than one minute without employing expensive lithographic methods. Design changes of fluidic channel can be made by ProE software, and which is applicable to rapid prototyping of microfluidic devices. The PDMS base and a glass cover slip were then assembled via the DSA. Before assembling the chip, glass cover was cleaned with ethanol using sonication. Then, it was washed with distilled water and dried under nitrogen gas. After cleaning steps, the glass cover was plasma treated for 60 seconds （100 mW, 1% 02, 60 s）. Next, specific antibody was pre-immobilized on the bottom of the microchannel and then bound with FITC-labeled detection antibodies to generate fluorescent signals. Then, the inlet with cell suspension solution was connected to a syringe pump which controlled the fluid velocity, flow rates was adjusted from 2 to 8/aL/min. Using this device, a high tumor cell capture efficiency of 92% was achieved at a flow velocity of 2 ~tL/min. In this paper, a new technique is described to reduce fabrication time and cost, and shows considerable promise for the translation to a rapid point-of-care diagnostic device for the detection of CTCs.
同期刊论文项目
同项目期刊论文
期刊信息
位置：