中文摘要：

采用显微注射方法,获得了转“全鱼”生长激素（GH）基因鲤鱼（转基因鱼）,应用抑制性差减杂交（SSH）技术,构建了4月龄F3转基因鱼胸腺发育差减cDNA文库,筛选并鉴定了与胸腺发育相关的81个与已知基因同源的表达序列标签（EST）。这些EST至少代表69个基因。根据基因的作用将其分为5类：18个与免疫和细胞防御有关;23个参与细胞生长、发育和分化等代谢过程;3个参与细胞信号传导和周期调控;8个在转录和表达调节中发挥作用;17个为核糖体蛋白基因,表明转基因鱼胸腺细胞处于活跃的蛋白合成状态。应用RT-PCR和虚拟Northern杂交技术进一步证实其中的若干基因在转基因鱼胸腺组织中的表达量增加。实验结果为阐明转植GH基因促进鲤鱼胸腺发育的相关分子机制提供了依据。

英文摘要：

＂All fish＂ growth hormone （GH） transgenic carps （transgenic carp） were obtained by the method of microinjection. By using suppression subtractive hybridization （SSH） techniques, a subtractive cDNA library of thymus in F3 degeneration of 4-month transgenic carp was constructed, and 81 differentialy expressed or up-regulation genes related to thymus development were isolated. By BLAST search, ESTs were identified as orthologs of known gene from other organisms. These identified clones were derived from at least 69 genes, which were categorized into 5 categories： 18 in cell defense/ immunity, 23 in metabolism including cell growth, development and differentiation, 3 in cell signal transduction and cell cycle regulation, 8 in regulation of transcription and expression, and 17 were ribosomal protein genes. The identification of 17 ribosomal protein genes indicated that the thymus tissue cells of transgenic carp were active in protein synthesis. RT-PCR and virtual Northern hybridization further demonstrated that the expression of several genes was upregulated in thymus of transgenic carps. The results were helpful to better understanding the molecular mechanism of transgene for GH in carps to promote thymus development.