中文摘要：

目的对半夏PinelliaeRhizoma及其炮制品姜半夏Pinelliae Rhizoma Praeparatumcum Zingibereet Alumine不同提取部位的HPLC特征指纹图谱进行研究，阐明半夏炮制前后主要药效物质变化。方法通过HPLC梯度洗脱建立半夏及其炮制品姜半夏的水及75％、95％乙醇提取部位的特征指纹图谱，并运用“中药色谱指纹图谱相似度评价系统”和SPSS17．0软件进行相似度和主成分分析（PCA）。结果分别建立了半夏及其炮制品姜半夏不同部位的HPLC特征指纹图谱共有模式，指认了肌苷、鸟苷、腺苷、琥珀酸、盐酸麻黄碱、6．姜辣素6个特征峰。姜半夏与半夏HPLC特征指纹图谱相比，姜半夏在保留时间18．3、73．5min附近新增2个峰（10号峰、19号峰6．姜辣素）。结论首次建立了半夏及其炮制品姜半夏不同提取部位的HPLC特征指纹图谱，该方法稳定、简便、可靠，可有效地控制半夏药材及姜半夏饮片的质量。

英文摘要：

Objective To study the characteristic fingerprint of different extract parts from Pinelliae Rhizoma （PR） and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine （PRPZA） by HPLC, to elucidate the major material foundations before and after processing, and to provide reliable method and scientific basis for the quality control of PR and PRPZA. Methods The HPLC fingerprints of water, 75% ethanol and 95% ethanol extracts from PR and PRPZA were established. The similarity was analyzed with ＂Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica＂. The principal component analysis （PCA） was performed by SPSS software 17.0. Results Six common modes of the HPLC characteristic fingerprint of different extract parts from PR and PRPZA have been established. Six specific peaks were identified as inosine, guanosine, adenosine, succinic acid, ephedrine hydrochloride, and 6-gingerol, respectively. Compared with the characteristic fingerprint of PR, there were two more peaks existed in retention time 18.3 （peak 10） and 73.5 min （peak 19, 6-gingerol） of PRPZA. Conclusion It is the first time to establish the HPLC characteristic fingerprint of different extract parts from PR and PRPZA. The method is stable, time-saving, and reliable, and could provide an efficient basis for the quality control of PR and PRPZA.