中文摘要：

目的研究肺癌细胞和人正常支气管细胞（human bronchial epithelial cell，HBE）中NF-kB2的表达情况及其启动子区CpG岛的甲基化状态。方法采用实时荧光定量PCR（Real-timePCR）方法检测肺癌细胞和正常支气管细胞NF-kB2基因mRNA的表达情况；采用Westernblot技术检测NF-kB2前体蛋白p100的表达情况；采用重亚硫酸盐测序（Bisulfite Sequencing PCR,BSP）技术检测NF-kB2启动子区CpG岛的甲基化状态。结果肺腺癌细胞株A549、肺鳞癌细胞株SK-MES-1和小细胞肺癌细胞株NCI-H446的NY-KB2mRNA的表达分别是HBE细胞株的6．42±0．91倍（P〈0.5，t=5．828）、2．78±0．52倍（P〈0．05，t=3．219）、2．79±0.33倍（P〈0．05，t=4．611）；3种肺癌细胞中p100蛋白较HBE细胞均上调表达；4种细胞株中所扩增片段的CpG位点均呈非甲基化状态。结论肺癌细胞及HBE细胞中NF-kB2启动子区域的CpG位点处于非甲基化状态．说明NF-kB2基因表达情况与甲基化状态无直接关系，其启动子区的甲基化修饰未直接参与该基因的表达调控，可能存在其他相关的调控机制，有待进一步的探索。

英文摘要：

To investigate the association between expression and methylation status of NF-kB2, we assessed the expression of NF-kB2 and also analyzed its methylation status in three kinds of lung cancer cell lines （A549, SK-MES-1, NCI-H446 ） and HBE cell line. Quantitative real-time PCR and Western blot were used to detect the expression of NF-kB2. In the same time, Bisulfite-sequencing PCR （BSP） was performed to detect the methylation status in the promoter region of NF-kB2. As a consequence, we found that both mRNA and protein expression of NF-kB2 in 3 lung cancer cell lines increased compared with HBE cell line. However, no methylation site of NF- kB2 was found in all the cell lines. Taken these findings together, we may conclude that overexpression of NF-kB2 in lung cancer cell lines is not directly regulated by its promoter methylation. Thus other related regulation mechanism of NF-kB2 overexpression in lung cancer cells should be taken into account further.