启动包裹无纺布的多微孔炭管为膜组件的膜曝气生物膜反应器(MABR),实现基于短程硝化和厌氧氨氧化的完全自养脱氮.首先接种普通硝化污泥启动反应器,在温度35℃,pH为7.9条件下,通过对膜内腔压力的适当控制逐步降低反应器溶解氧浓度,实现亚硝酸盐的积累.然后再次接种厌氧氨氧化污泥,使无纺布上形成好氧氨氧化菌与厌氧氨氧化菌稳定共存的膜曝气生物膜,从而实现全程自养脱氮.结果表明,经过120 d连续运行,在膜内压力为0.015 MPa,水力停留时间6 h,进水NH4^+-N为200 mg/L±10 mg/L条件下,NH4^+-N转化率达到88.7%,出水总氮平均为48.65 mg/L,总氮去除率达到83.77%.荧光原位杂交(fluorescent in situ hybridization,FISH)分析表明,好氧氨氧化菌(AOB)和厌氧氨氧化菌作为主要功能菌群分别控制着靠近炭管膜/生物膜界面区域和靠近生物膜/液体界面区域.
A laboratory-scale membrane-aerated biofilm bioreactor (MABR) equipped with nonwoven fabrics support around the gas-permeable carbon tube was developed for single-stage autotrophic nitrogen removal based on partial nitrification and anaerobic ammonium oxidization. Initially, the reactor was inoculated with nitrifying biomass. By decreasing the air pressure in the lumen of carbon tube step by step, We can obtain stable nitrite accumulation. Subsequently, the reactor was reinoculated with Anammox biomass to construct a quite stable biofilm system for completely autotrophic N removal, After 120 d of operation, 88.7% of NH4^+ -N removal rate, 48.65 mg/L of average TN in the effluent, 83.77 % of maximal TN removal efficiency was achieved under the following conditions: air pressure of 0.015 MPa, HRT of 6 h, and influent NH4^+ -N of 200 mg/L ± 10 mg/L.