中文摘要：

目的探讨肿瘤增殖型腺病毒Ad-delE1b55KD-shRNA／survivin-EGFP介导的针对survivin的高选择性RNA干扰对在体结肠癌细胞HT-29增殖和凋亡的影响。方法用携带针对人survivin的shRNA的肿瘤增殖型腺病毒Ad-delE1b55KD-shRNA／survivin-EGFP转染结肠癌细胞HT-29裸鼠移植瘤模型，以携带相同shRNA的复制缺陷型腺病毒和脂质体载体为对照，检测移植瘤体变化，以及TUNEL法检测肿瘤细胞凋亡率。结果Ad-delE1b55KD-shRNA／survivin-EGFP介导的高选择性RNA干扰使结肠癌裸鼠移植瘤的瘤体减小、移植瘤细胞凋亡率显著增加，且高于目前常用的复制缺陷型腺病毒载体和脂质体载体（P〈0．05）。结论Ad-delE1b55KD-shRNA／survivin-EGFP介导的高选择性RNA干扰能高效地诱导结肠癌细胞凋亡并抑制其增殖。

英文摘要：

Objective To investigate the effect of RNAi-mediated survivin gene with conditionally replicating adenovirus Ad-de- 1-shRNA/survivin-EGFP silencing on propagation and apoptosis of colon carcinoma cell lines HT-29. Methods Ad-de- lE1b55KD-shRNA/survivin-EGFP was transfected to transplantation tumor on athymic mouse,replication defective adenovirus and liposome vector which was contained the same shRNA as Ad-delE1b55KD-shRNA/survivin-EGFP were as control one. Then the change of transplantation tumor on athymic mouse was tested,and TUNEL was used to assay the apoptosis rate of transplantation tumor cells. Results After transfection of Ad-delE1b55KD-shRNA/survivin-EGFP,the quality of transplantation tumor on athymic mouse were reduced and the apoptosis rate of transplantation tumor cells were higher than replication defective adenovirus and Iipo- some vector which was contained the same shRNA（P〈0.05）. Conclusion The effect of Ad-delE1b55KD-shRNA/survivin-EGFP to colon carcinoma cell on propagation and apoptosis were higher than replication defective adenovirus and liposome vector.