中文摘要：

以秀丽隐杆线虫模式生物为研究对象，研究大豆异黄酮染料木素微生物代谢产物左旋-5-羟基-雌马酚对线虫寿命、生殖能力以及抗热应激和抗氧化应激能力的影响。研究结果表明，浓度为0．1 mmol／L和0．2 mmol／L的左旋．5一羟基．雌马酚不仅能够显著延长线虫寿命，同时还能显著增强线虫耐热和抗氧化应激能力，而在相同浓度下线虫生殖能力则无明显不良影响。通过进一步对比分析转录物水平以及喂食相应线虫突变体daf-16（mu86）和daf-2（e1370），确定左旋-5-羟基．雌马酚延长线虫寿命与DAF-2／DAF．16 Insulin／IGF-1信号转导通路相关。有关左旋-5-羟基．雌马酚显著延长线虫寿命和增强线虫抗性为国内外首次报道。

英文摘要：

Caenorhabditis elegans （C. elegans） was used as an animal model to study the effect of （-）-5-hydroxy-equol, a microbialmetabolite of isoflavone genistein, on the lifespan, fecundity and resistance against thermal and oxidative stress. The resultsshowed that （-）-5-hydroxy-equol not only significantly increased the lifespan of C. elegans but also significantly enhancedthe resistance against thermal and oxidative stress at the concentrations of 0.1 mmol/L and 0.2 mmol/L. However, the fecundityof C. elegans was not obviously influenced after being exposed to the same concentrations of （-）-5-hydroxy-equol. Further studieson comparative transcriptome analyses and the lifespan ofdaf-16 （mu86） mutant and daf-2 （e1370） mutant indicated that（-）-5-hydroxy-equol prolonged the lifespan of C. elegans through DAF-2/DAF-16 Insulin/IGF-1 signaling pathway. This isthe first report that （-）-5-hydroxy-equol was able to increase the lifespan and improve the thermal and oxidative stress toleranceof C. elegans.