中文摘要：

目的：比较不同消毒方法对壳聚糖温敏凝胶物理性能及生物学特性的影响。方法：采用常规消毒法（壳聚糖-盐酸液高温高压消毒）和改进消毒法（壳聚糖粉单独高温高压消毒）制备壳聚糖温敏凝胶,分别检测其凝胶化时间、粘度;通过扫描电镜观察2组壳聚糖温敏凝胶的超微结构;在2组壳聚糖温敏凝胶表面培养第三代人牙周膜细胞,倒置荧光显微镜观察凝胶表面细胞活性;用2组壳聚糖温敏凝胶浸提液培养第三代人牙周膜细胞,MTT法检测细胞增殖情况。结果：改进组壳聚糖温敏凝胶凝胶化时间5～6 min稳定,常规组壳聚糖温敏凝胶凝胶时间为10～30 min不等;改进组的壳聚糖温敏凝胶初始粘度为（9.94±0.38）Pa.s,在37℃环境下其粘度随时间延长而增加,10 min后达（50.25±0.96）Pa.s,此后趋于平稳。常规组初始粘度为（0.90±0.45）Pa.s,其粘度随时间延长增加不明显,15 min时仅为（4.05±0.72）Pa.s,2组各时间点间均有显著差异（P〈0.05）;扫描电镜观察2组壳聚糖温敏凝胶均呈三维立体网状多孔结构,常规组孔径为10～48.33μm,改进组孔径为0.385～2μm。人牙周膜细胞不仅在2组壳聚糖温敏凝胶表面生长良好,而且在其浸提液中也均增殖正常。结论：改进消毒方法制备的壳聚糖温敏凝胶凝胶化时间更短,粘度更大,与常规消毒方法一样具有良好的生物相容性。

英文摘要：

AIM： To observe and compare the physical properties and biocompatibility of chitosan thermosensitive gel treated with two different sterilization methods.METHODS：The gelation time and viscosity of chitosan thermosensitive gel were prepared by autoclaving chitosan solution（conventional sterilization） or chitosan powder（improved sterilization）.Gelation time and viscosity of the gels were evaluated respectively.The Ultrastructure of chitosan gel was visualized by scanning electron microscopy.The third generation periodontal ligament cells were incubated on the chitosan gel,the cell activity on gel surface was observed by inverted fluorescence microscope.The third passage periodontal ligament cells were incubated in the chitosan thermosensitive gel extracts,the proliferation of cells was measured by MTT assay.RESULTS： The gelation time of the improved gel was 5-6 min and the chitosan was more stable,while the gelation time of the conventional gel was 10-30 minutes.The initial viscosity of the improved group was（9.94±0.38）Pa.s.The viscosity increased with time at 37 ℃;and at 10 min,it reached the maximum value of（50.25±0.96）Pa.s,thereafter remained stable.The initial viscosity of conventional group was（0.90±0.45）Pa.s.The viscosity increased slightly,and at 15 min the value was（4.05±0.72）Pa.s.There was significant difference between the two groups at each time point.Three-dimensional mesh porous structures were observed by scanning electron microscope in both groups.The pore size was between 10 μm and 48.33 μm in conventional group,compared to 0.385 μm and 2 μm in improved group.Human periodontal ligament cells prolifereated equally well on surfaces of both gels.CONCLUSION：Chitosan thermosensitive gel can be prepared by the improved method with shorter time while showing the same biocompatiblility with the gel prepared by conventional method.