中文摘要：

合成了6种新的双胍类金属配合物．采用红外光谱、核磁共振光谱确定了配合物结构．测定了不同R值下（R=CDNA／C配合物）金属配合物ct—DNA体系的紫外吸收光谱，研究了配合物与ct—DNA的作用，并计算出6种配合物的结合常数Kb值，分别为1．10×10^4，1．34×10^4，1．05×10^4，1．23×10^4，1．33×10^4和1．42×10^4．应用琼脂糖凝胶电泳研究了配合物与pBR322DNA的作用关“系，结果表明：配合物均与ct—DNA有一定的作用，且随着ct-DNA浓度增加逐渐增加，紫外吸收逐渐减弱，说明两者之间的作用方式为嵌入键合；配合物对pBR322DNA均具有不同程度的切割作用，且浓度越大切割作用越明显；金属离子相同时，配合物结构空间位阻越小，越有利于与DNA之间的作用；配体相同时，金属离子的中心离子缺电子程度越大，其配合物与DNA发生相互作用的趋势越强，可能与配合物和电负性较大的DNA碱基发生电相互作用有关．

英文摘要：

Abstract. Six kinds of biguanidine-metal complexes were synthesized, and characterized by IR, 1 H NMR and 13C NMR spectra. The interaction of the six complexes with calf thymus DNA was studied by the UV-visible spectra. The spectra showed hypochromism as the increasing of the ct-DNA, which suggested that the combination of the metal complexes and ct-DNA was caused by the intercalation. The binding constants of the six complexes Kb, were calculated, resulting as 1.10×10^4 , 1.34 ×10^4, 1.05 ×10^4 , 1. 23 ×10^4 , 1. 33 ×10^4 and 1.42 ×10^4 for complexes Ⅰ-Ⅵ. The value indicated that the comp Ⅵ, having the proper structure, showed the stronger binding ability to DNA than the other five complexes. The Gel electrophoresis showed that as the increase of the complexes, the cleavage of pBR322 DNA was more obvious. The results also prompted that the more beneficial to interaction with DNA, the smaller space factor of the complexes structure for the same metal ions and the greater metal ion radius of the same ligand. These may be related to complexes regulation of the ability of the structure.