对比研究了空气CO2浓度与模拟烟道气CO2浓度〔15%(v/v)〕对小球藻(Chlorella sorokiniana)生长和重要生化组成变化规律的影响。结果表明,15%CO2浓度条件下,小球藻生长速率、干物质总量、细胞密度均高于空气CO2浓度培养条件下的相应指标,其生物质积累主要是细胞内碳水化合物含量增加的结果。空气CO2浓度下,生物质积累是细胞内蛋白质和碳水化合物含量同时增加的结果。叶绿素测定结果显示,小球藻叶绿素含量对高浓度CO2响应较快,叶绿素(Chl a+b)含量在培养2 d时迅速升高至干重的3.5%,且叶绿素a/b比值在初始通气3 d显著升高。在快速生长初期,15%CO2浓度培养的小球藻光能转化效率高于空气CO2浓度条件的转化效率。
In this study,Chlorella sorokiniana was cultured under aerating 15% CO2 and ambient CO2,respectively.The effect of different CO2 concentration on the relationship among cell growth,the variation of important biochemical compositions as well as protein and carbohydrate content were investigated.The results demonstrated that the rate of cell growth,dry biomass accumulation and cell density of C.sorokiniana under 15% CO2 were all higher than those under ambient CO2.The biomass accumulation of C.sorokiniana under 15% CO2 was mainly due to increased carbohydrates.But for cells grown under ambient CO2,C.sorokiniana showed lower cell division and higher dry weight per cell.Chlorophyll measurement results show that The chlorophyll exhibited a rapid response to high concentration of CO2,and the chlorophyll a and b contents of C.sorokiniana based on dry weight rise to 3.5% within 2 days under 15% CO2.Similarly,the ratio of chlorophyll a and b of C.sorokiniana under 15% CO2,was also higher than that under ambient CO2 for the first 3 days,indicating that the light energy conversion efficiency of algae cultured in 15% CO2 was higher than that cultured in ambient CO2.