染色体微切割与微克隆技术首先在动物和人类上得到应用,随着PCR技术的发展,该技术在植物遗传与进化研究等方面也得到了较为广泛应用。本文就植物染色体显微切割和微克隆技术的原理、主要操作规程以及在植物中应用的研究现状、存在问题进行了综述。
Chromosome microdissection and microcloning technology was first used in animal and human. With the development of PCR,they was applied in plant genetic and evolutional researches widely. This article summarized the principle of the technology, its major procedures application situations, and current troubles.