中文摘要：

以对羟基苯甲酸甲酯为原料，通过硝化，还原，关环，甲基化合成2-（甲硫基）苯并[d]噁唑5-羧酸，过程的总收率为49．17％．利用红外光谱（FTIR）、核磁共振谱（^1HNMR）、质谱（HPLC—MS）和元素分析等分析手段对中间产物及目标化合物进行了表征．通过分子自组装膜法将其负载在金电极上，分别在四丁基六氟膦酸胺-乙腈电解液、磷酸盐缓冲水溶液中检测其电化学发光性能，结果表明，在水相中电致化学发光强度明显高于有机相．

英文摘要：

In this paper, with methyl 4-hydroxybenzoate as raw material, 2-（methylthio）benzo [d]oxazole -5- carboxylic acid was synthesized by nitration , reduction, cyclization, methylation. The structures of the intermediates and 2-（methylthio）benzo [d]oxazole-5-carboxylic acid were characterized by FFIR, 1HNMR, HPLC-MS and element analysis. The product was loaded on the surface of gold elctrode by self-assembly, and the electrochemiluminesoence was measured in tetrabutylammonium hexafluorophosphate-acetonitrile electrolyte and phosphate buffer solution. The results reveal that electrochemiluminesoence intensity is higher in aqueous phase than it is in organic phase.