在单因素的基础上,根据中心组合(Box-Behnken)试验设计原理,采用4因素(提取时间、乙醇浓度、料液比、pH)3水平的响应面分析方法,建立二次多项式回归方程的预测模型,研究各变量交互作用对紫红肉火龙果果肉色素提取的影响。结果表明,紫红肉火龙果果肉色素提取的最优工艺条件为提取时间61 min、乙醇浓度27%、料液比1∶55、pH 5.3。
Based on the results of single factor experiments,Box-Behnken central composite design(CCD) involving four variables(extraction time,ethanol concentration,material/liquid ratio and pH) at three levels was applied to set up a quadratic polynomial regression equation for pigment extraction yield,and the pariwise interactiions of the above four variables on extraction rate of pigment from red pitaya flesh were investigated by response surface analysis.Results showed that the optimal conditions for extracting pigment from red pitaya flesh were found to be extraction time of 61 min,ethanol concentration of 27%,the ratio of material to solvent of 1∶55(g/mL) and pH 5.3.