中文摘要：

目的：从线粒体角度探讨电针治疗阿尔茨海默病（AD）的作用机制。方法：选取8月龄SAMP8小鼠12只,随机分为模型组6只,电针组6只,另选取6只8月龄的SAMR1小鼠为对照组。电针组电针＂百会＂＂大椎＂＂肾俞＂＂太溪＂穴,每日1次,每次20 min,10次为一疗程,共治疗3个疗程;其他两组不做治疗干预,只进行相同方式、相同时间和相同程度的抓取和小鼠固定器固定。疗程结束后,采用Morris水迷宫检测小鼠学习记忆能力,分光光度法检测海马线粒体呼吸链酶复合物的活性,反相高效液相色谱法检测细胞三磷酸腺苷（ATP）水平。结果：与对照组相比,模型组小鼠平均逃避潜伏期明显延长,原平台象限停留时间缩短,海马线粒体呼吸链酶复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性降低,细胞ATP含量减少;与模型组比较,电针组平均逃避潜伏期缩短,原平台象限停留时间延长,海马线粒体呼吸链酶复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性升高,细胞ATP含量增加。结论：电针能够提高小鼠海马线粒体呼吸链酶复合物活性及细胞ATP含量,改善线粒体功能,可能是治疗AD的机制之一。

英文摘要：

Objective To investigate the mechanism of electroacupuncture （EA） in treating Alzheimer＇s disease （AD） from aspect of mitochondria. Methods Twelve 8-month old SAMP8 mice were randomly divided into a model group （Group Mod, n=6） and an electroacupuncture group （Group EA, n=6）, and six 8-month old SAMR1 mice were selected as a control group （Group Cont）. Animals in Group EA was treated with EA at ＂Baihui＂ （百会 GV 20）, ＂Dazhui＂ （大椎 GV 14）, ＂Shenshu （肾俞 BL 23） and ＂Taixi＂ （太溪 KI 3） for 20 min, once daily with 20 days of treatments as a therapeutic course, lasting for 3 courses. Mice in the other two groups were fixed in the same way as those in Group EA at the same time without any treatment. After treatment, learning and memory abilities of the mice were measured by Morris water maze, activities of enzyme complex of hippocampal mitochondrial respiratory chain were measured by spectrophotometry, and levels of adenosine triphosphate （ATP） were detected by reverse phase high performance liquid chromatography （HPLC） method. Results Compared with Group Cont, the average escape latency in Group Mod was significantly prolonged, the residence time on the platform quadrant was shortened, the activities of enzyme complex Ⅰ, Ⅱ, Ⅲ and Ⅳ in hippocampal mitochondrial respiratory chain was decreased, and ATP contents was lessened in Group Mod. Compared with Group Mod, the average escape latency was significantly shortened, the residence time on the platform quadrant was lengthened, the activities of enzyme complex Ⅰ, Ⅱ, Ⅲ and Ⅳ in hippocampal mitochondria respiratory chain were significantly increased, and ATP contents were also increased in Group EA. Conclusion Electroacupuncture can elevate the activities of enzyme complex in hippocampal mitochondrial respiratory chain and ATP contents, and improve mitochondrial functions, which may be one of the underlying mechanisms of EA in treatment of AD.