中文摘要：

目的探讨转化生长因子β1（transforming growth factor-β1, TGF-β1）对口腔鳞癌及腺样囊性癌细胞中纤维黏连蛋白（fibronectin，FN）基因EDA片段的调节作用。方法应用免疫组织化学及半定量逆转录聚合酶链反应法（RT-PCR），分别从蛋白及mRNA水平观察口腔鳞癌及腺样囊性癌高、低转移潜能细胞中加入外源性TGF-β1后FN基因EDA片段的表达变化情况。结果3株细胞加入TGF-β1组与未加TGF-β1组EDA片段蛋白表达阳性细胞率比较显示，口腔鳞癌细胞株（Tca83）阳性细胞率由15．4±4．1增加到50．7±10．5，差异有统计学意义（P〈0．01），腺样囊性癌低转移株（SACC-83）阳性细胞率由71．9±4．8增加到86．1±5．5，差异也有统计学意义（P〈0．05），但腺样囊性癌高转移株（SACC-LM）阳性细胞率由93．3±2．4增加到94．1±3．4，差异无统计学意义（P〉0．05）。3株细胞加入TGF-β1组EDA^＋ mRNA表达较未加TGF-B1组明显升高，而EDA-mRNA的表达下降。且差异均有统计学意义（P〈0．05）。结论高浓度TGF-β1可以影响口腔鳞癌及腺样囊性癌细胞中FN基因EDA片段的剪切并促进EDA片段的表达，可能成为影响肿瘤细胞黏附能力及肿瘤侵袭和转移的相关因素。

英文摘要：

Objective To investigate the effect of transforming growth factor-β1 （TGF-β1） on EDA region of fibronectin in oral squamous cell carcinoma and adenoid cystic carcinoma cells. Methods Immunohistochemistry and reverse transcription polymerase chain reaction technique were used to detect the changes of EDA proteins and mRNAs expression. Results The immunostaining ratio in Tca83 cells with TGF-β1 was greatly higher than that in Tca83 cells without TGF-β1 （ P 〈 0. 01 ）, the immunostaining ratio in SACC-83 cells with TGF-β1 was higher than that in SACC-83 cells without TGF-β1 （P 〈 0. 05）, but there was no significant difference between SACC-LM ceils with TGF-β1 and SACC-LM cells without TGF-β1 （P 〉 0. 05）. In the three oral carcinoma cells, the expression of EDA^＋ mRNAs in the group with TGF-β1 was higher than that in the group without TGF-β1 （ P 〈 0. 05 ）. The expression of EDA - mRNAs in the group with TGF-β1 was lower than that in the group without TGF-β1 （ P 〈 0. 05 ）. Conclusions TGF-β1 could influence EDA region splicings and upregulate the expression of EDA region in Tca83 ,SACC-83 and SACC- LM cells, and might play an important role in accelerating oral carcinoma cells adhension and tumor invasion and metastasis.