中文摘要：

没有压制房间生长，在 microalgae 房间导致类脂化合物累积对从 microalgae 的 biodiesel 的节俭的生产重要。在二个实验，我们证明海洋的 microalgae Isochrysis galbana 的细胞集中和类脂化合物内容在生长媒介取决于铁集中。在实验我，到在晚指数的生长阶段的媒介的增加的 chelated FeCl3 延长了这个阶段并且增加了类脂化合物内容在我。galbana 房间。最后的房间密度和类脂化合物内容我。与 chelated FeCl3 补充的 galbana 分别地比非补充的文化的高约 2 和 1.65 倍。在实验 II，我。在晚指数的阶段的 galbana 房间被收集并且重新接种进在各种各样的集中包含 Fe3+ 的新媒介。最后的房间集中和类脂化合物内容在最高的铁集中被最大化(由在 1.2 点的干燥重量的 38% 生物资源 ? 桴 ? 潦吗？

英文摘要：

Inducing lipid accumulation in microalgae cells without suppressing cell growth is vital to the economical production ofbiodiesel from microalgae. In two experiments, we demonstrate that the eel1 concentration and lipid content of marine microalgae Isochrysis galbana depend upon the iron concentration in the growth media. In Experiment I, adding chelated FeC13 to the medium at the late exponential growth phase prolonged this phase and increased the lipid content in I. galbana cells. The final cell density and lipid content of I. galbana supplemented with chelated FeC13 was approximately 2 and 1.65 times higher than that of non-supplemented cultures, respectively. In Experiment II, I. galbana cells in the late exponential phase were collected and re-inoculated into new media containing Fe3＋ at various concentrations. The final cell concentration and lipid content were maximized at the highest iron concentration （38% biomass by dry weight at 1.2×10^-5 mol/L Fe3＋）. In this study, intracellular neutral lipid storage was evaluated by fluorescent spectrophotometry using fluorochrome Nile red, and the measurement conditions were optimized.