目的 测定马兰中多糖含量,为马兰的应用和质量评价提供科学依据.方法 以葡萄糖为对照品,采用苯酚-硫酸比色法,在490 nm波长下测定多糖含量.结果 不同产地、采收时间的马兰多糖含量差异显著.结论 苯酚-硫酸比色法操作简单,重复性好,结果准确可靠,可用于马兰中多糖的含量测定.
Objective To determine the content of polysaccharides in Kalimeris indica (Linn.) Sch. from different areas and to provide a scientific basis for the application and quality evaluation of Kalimeris indi- ca (Linn.) Sch. Methods With glucose as a control, the content of polysaccharides in Kalimeris indica (Linn.) Sch. from different areas was determined by phenol-sulfuric acid colorimetry at a wavelength of 490 nm. Results The content of polysaccharides varied significantly in Kalimeris indica (Linn.) Sch. pro- duced in different areas and harvested at different times. Conclusion This determination method has the advantages of easy operation, good repeatability, and accurate results and is suitable for content determi- nation of polysaccharides in Kalimeris indica (Linn.) Sch. from different areas.